阿奇霉素与茜素的电荷转移反应及其测定  

Spectrophotometric Determination of Azithromycin Based on the Charge Transfer Reaction between Azithromycin and Alizarin

作　　者：李华侃[1] 赵延清[1] 王玉华[1] 康剑峰[1] 

机构地区：[1]锦州医学院,锦州121001

出　　处：《分析化学》2004年第5期598-600,共3页Chinese Journal of Analytical Chemistry

摘　　要：建立了一种快速测定阿奇霉素的荷移分光光度法。阿奇霉素与茜素在乙醇介质中发生电荷转移反应 ,电荷转移络合物在 5 4 6nm处有最大吸收 ,表观摩尔吸光系数为 5 .79× 10 3 L·mol-1·cm-1;该络合物的组成是 1∶1;稳定常数为 4 .8× 10 3 ;药物浓度在 5～ 12 0mg/L范围内服从比耳定律。利用本法测定了阿奇霉素制剂中有效成分的含量 ,并与文献法进行比较 ,二者结果基本吻合。A spectrophotometric method for the determination of azithromycin has been developed based on the charge transfer reaction between azithromycin as donor and alizarin as acceptor in ethanol solution. The composition ratio and stability constant of charge transfer complex are 1:1 and 4.8 x 10(3) respectively. The apparent molar absorptivity of complex at 546 nm is 5.79 x 10(3) L . mol(-1) . cm(-1). Beer's law is obeyed in the range of 5 similar to 120 mg/L azithromycin. The relative standard deviation of six determinations is 1.4%. The proposed method has been applied to the determination of azithromycin in preparation with satisfactory results.

关 键 词：阿奇霉素 茜素 电荷转移反应 测定方法 分光光度法 大环内酯抗生素 

分 类 号：TQ465[化学工程—制药化工] O657.3[理学—分析化学]