荧光法研究秋水仙碱和牛血清白蛋白的相互结合作用  

Study on the Interaction between Colchicine and Bovine Serum Albumins by Fluorescence Method

作　　者：刘永明[1] 李桂芝[1] 孙希芬[1] 

机构地区：[1]烟台大学化学院,烟台264005

出　　处：《分析化学》2004年第5期615-618,共4页Chinese Journal of Analytical Chemistry

摘　　要：用荧光光谱法、分光光度法研究了水溶液中秋水仙碱与牛血清白蛋白 (BSA)的相互结合反应。研究表明 :二者以摩尔比 1∶1牢固结合 ,其平衡常数K0 =1.92× 10 5L/mol。根据F rster非辐射能量转移机理 ,求算了给体 (BSA)与受体 (秋水仙碱 )间距离r =3.6 3nm和能量转移效率E =0 .17。实验表明The binding reaction between colchicine and bovine serum albumins (BSA) in aqueous solution was studied by fluorescence and ultraviolet-visibale absorption spectra. The results indicates that colchicine strongly binds to BSA with a molar ratio of 1:1 and the equilibrium constant K-0 is 1.92 x 10(5) L/mol. The Stern-Volmer curve on the fluorescence of BSA quenched by series of colchicine concentrations shows good linear relationship, which illustrates the combination reaction of colchicine and BSA is a single static quenching process. The shortest binding distance ( r = 3.63 nm) and energy transfer efficiency (E = 0.17) between donor (BSA) and acceptor (colchicine) were obtained by Foster's nonradiative energy transfer mechanism.

关 键 词：荧光法 秋水仙碱 牛血清白蛋白 荧光猝灭反应 结合反应 药物 血浆蛋白 生物碱 

分 类 号：O657.3[理学—分析化学]