戊唑醇和三唑酮对映体的手性拆分  

The Enantiomeric Separation of Tebuconazole and Triadimefon

作　　者：王鹏[1] 江树人[1] 张宏军[1] 周志强[1] 

机构地区：[1]中国农业大学理学院应用化学系,北京100094

出　　处：《分析化学》2004年第5期625-627,共3页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No .2 0 3 770 5 2 )资助项目

摘　　要：以纤维素 三 ( 3,5 二甲基苯基氨基甲酸酯 ) (CDMPC)高效液相色谱手性固定相 (CSP)成功地拆分了戊唑醇和三唑酮对映异构体 ,通过考察流动相中异丙醇的含量、柱长及温度对手性拆分的影响 ,优化色谱分离条件。结果显示 :该固定相对两种手性农药有较好的拆分效果 ,使用 2 5 0mm色谱柱 ,流动相中含 5 %异丙醇时戊唑醇得到最佳分离 ,分离度为 1.2 2 ;三唑酮在异丙醇含量为 2 %时有最大分离度 1.4 7。减少异丙醇含量对两种农药均有利于增大分离度 。The racemates of tebuconazole and triadimefon were resolved on the cellulose-tris(3,5-dimethylphenylcarbamate) (CDMPC) high-performance liquid chromatography chiral stationary phase (CSP). By investigating the influences of the percentage of iso-propanol in the mobile phase, the column length and temperature on the resolution and the chromatographic conditions were optimized. The results showed that the CSP exhibited good stereoselectivity to the two chiral pesticides. Tebuconazole and triadimefon obtained the best resolution of 1.22 and 1.47 with 5% and 2% iso-propanol in mobile phase respectively using 250 mm column. When the percentage of iso-propanol in mobile phase decreased, the resolution factor of the two chiral pesticides both increased. Low temperature was good for the separation of triadimefon and tebuconazole.

关 键 词：戊唑醇 三唑酮 对映体 手性拆分 手性固定相 温度 液相色谱分析 手性农药 三唑类杀菌剂 

分 类 号：O657.7[理学—分析化学]