应用遗传毒理学方法对表皮生长素的致突变性和皮肤毒性研究  

Study on the mutagenicity and skin toxicity of the HEGF through genetic toxicology tests

作　　者：许欣欣[1] 邓丽霞[2] 邓文[1] 安蔚[1] 万惠香[1] 

机构地区：[1]泰山医学院,山东泰安271000 [2]中山医科大学公共卫生学院,广东广州510089

出　　处：《泰山医学院学报》2004年第1期35-38,共4页Journal of Taishan Medical College

摘　　要：目的　为临床安全使用表皮生长素药物提供科学参考依据。方法　应用Ames试验、CHL细胞染色体畸变试验、小鼠骨髓细胞微核试验、小鼠精子畸形试验、豚鼠皮肤致敏试验及家兔皮肤、眼刺激试验等方法评价表皮生长素的致突变性和皮肤毒性。结果　以上测试方法均呈现阴性结果。结论　通过遗传毒理学和皮肤毒性测试方法未观察到表皮生长素的致突变性和皮肤毒性效应。Objective: To provide evidence for the safe use of HEGF(recombinant Human epidermal growth factor) in clinical practice. Methods: The mutagenicity and skin toxicity of the HEGF were studied through different tests: the Ames assay, the CHL cell chromosome analysis, the mouse marrow cell MNT, the mouse sperm teratogenicity, the guinea pig passive cutaneous anaphylaxis and rabbit skin and eyes stimulating tests. Results: Negative results were shown in all the tests. Conclusion: No mutagenicity or skin toxicity effect of HEGF on be observed through genetic toxicology and skin toxicity tests.

关 键 词：遗传毒理学方法 表皮生长素 致突变性 皮肤毒性 

分 类 号：R114[医药卫生—卫生毒理学]