极低频电磁场对中脑神经干细胞体外诱导分化的影响  

Effects of extremely low frequency electromagnetic fields on the induction and differentiation in vitco ofmidbrain neural stem cells

作　　者：邢萱[1] 李怡[2] 王小平[3] 李蕾[1] 滕海英[1] 

机构地区：[1]解放军第二军医大学数理教研室,上海市200433 [2]解放军第二军医大学神经生物学教研室,上海市200433 [3]武汉理工大学统计教研室,湖北省武汉市430063

出　　处：《中国临床康复》2004年第13期2458-2459,T001,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察极低频电磁场(extremelylowfrequencyelectromagneticfield,EMF)对新生大鼠中脑神经干细胞神经元分化的影响。方法:将新生大鼠中脑神经干细胞分别置于5Hz和20Hz极低频正弦交变磁场(8mT)中诱导分化,每天上午、下午各一次,15min/次,分别作用1d、5d或10d,并设立相应的对照组。利用神经元特异性标记物MAP2抗体进行免疫荧光染色,计数MAP2阳性细胞的百分比。结果:5Hz和20Hz极低频正弦交变磁场的干预,神经干细胞(neuralstemcells,NSC)分化比例明显增加。20Hz磁场刺激1d,可增加NSC神经元的比例在连续10d的磁场干预后效果最显著(P<0.01)达到47%±99%。结论:5Hz和20Hz极低频正弦交变磁场以不同的效应模式促进NSC神经元向的分化,极低频正弦交变磁场可以成为NSC定向分化研究的重要手段之一。AIM:To observe the effect of extremely low frequency electromagnetic field (EM F) on the neuron-orientated differentiation of neural stem cells(NSCs) from mid brains of new born rats. METHODS:NSCs were exposed to extremely low-frequency sinusoidal alternating E MF(8 mT) with the frequency of 5 Hz and 20 Hz for 15 minutes respectively in the morning and afternoon totally for 1,5 or 10 days in order to induce differentia tion.Corresponding control group was established.Neuron-specific marker MAP2 an tibody was used for immunofluorescent staining, and then the percentage of MAP2 positive cells was calculated. RESULTS:The differentiated percentage of NSCs increased when the NSCs were exp osed to EMF with the frequency of 5 Hz and 20 Hz.After exposure to 20 Hz EMF for 1 day, the percentage of NSCs increased,and reached the peak(47%±99%) after 10 days(P< 0.01). CONCLUSION:20 Hz and 5 Hz EMFs promote the differentiation of midbrain NSCs to a neuronal phenotype in different ways,suggesting that extremely low-frequency sinusoidal alternating EMF can be an important way to study the differentiation of NSCs.

关 键 词：极低频电磁场 中脑神经干细胞 细胞分化 新生大鼠 细胞计数 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]