大鼠局灶性脑缺血耐受中即早基因c-jun表达的变化  

Expression of immediate early gene c-jun in rats with focal cerebral ischemic tolerance

作　　者：邓艳秋[1] 赵纲[2] 赵政[3] 

机构地区：[1]华中科技大学同济医学院病理生理教研室,湖北省武汉市430030 [2]哈尔滨医科大学病理教研室,黑龙江省哈尔滨市150086 [3]哈尔滨工业大学计算机科学与技术学院,黑龙江省哈尔滨市150001

出　　处：《中国临床康复》2004年第13期2474-2475,T002,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:研究即早基因c-jun在大鼠短暂局灶性脑缺血预处理诱导脑梗死保护中的表达。方法:采用开颅方法阻断大鼠大脑中动脉(MCAO),通过脑梗死体积分析及病理形态学变化,观察脑缺血预处理的保护作用。采用原位杂交和免疫组化方法观察即早基因c-jun在脑缺血耐受中的表达。结果:预处理未引起脑组织神经元的病理性损伤,未经预处理的缺血组2,6和24h的脑梗死体积为(59.23±10.50),(72.35±12.30),(135.26±13.21)mm3,经预处理的脑缺血组2,6和24h脑组织梗死体积显著减小分别为(38.35±11.2),(51.25±13.46),(83.25±14.60)mm3,缺血预处理诱导了再次缺血后胶质细胞c-junmRNA及蛋白的早期表达。结论:短暂局灶性脑缺血预处理能够诱导对脑梗死的保护作用;即早基因表达的改变可能与脑缺血预处理诱导脑缺血耐受有关。AIM:To study the expression of immediate early gene c-jun during transient fo cal ischemic preconditioning(IPC) against cerebral infarction(CI). METHODS:After middle cerebral artery occlusion(MCAO) was conducted in rats for IPC,the protective role of IPC was evaluated by analyzing infarct volume and pa thomorphological changes.Expression of immediate early gene c-jun in ischemic t olerance was determined with in situ hybridization and immunohistochemical metho d. RESULTS:IPC did not result in obvious pathological damage of neurons in brain tissue.Infarct volumes in the ischemic group without IPC were(59.23±10.50),(72. 35±12.30) and(135.26±13.21) mm3 respectively 2,6 and 24 hours after MCAO,signi ficantly larger than those in the IPC group[(38.35±11.2),(51.25±13.46) and(83. 25±14.60) mm3 respectively].IPC rats exhibited early expression of c-jun mRNA and protein in glial cells after secondary ischemia. CONCLUSION:IPC is effective in protecting CI,and PC-induced ischemic toleranc e is associated with the changes in the early expression of immediate early gene c-jun in astroglia.

关 键 词：大鼠 局灶性脑缺血 即早基因 C-JUN 基因表达 脑梗死 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R394[医药卫生—临床医学]