电针对大鼠海马兴奋性突触后电位长时程增强的作用(英文)  

Effects of electrical acupuncture on long-term potentiation of excit atory postsynaptic potential in rat hippocampus

作　　者：马骋[1] 闫丽萍[1] 沈梅红[1] 

机构地区：[1]南京中医药大学江苏省针灸学重点实验室,江苏省南京市210029

出　　处：《中国临床康复》2004年第13期2587-2589,共3页Chinese Journal of Clinical Rehabilitation

基　　金：江苏省中医药管理局基金项目(970101)~~

摘　　要：背景:海马长时程增强(long-termpotentiation,LTP)与行为学研究相结合,已被广泛用于包括中医药在内的改善学习记忆功能和治疗老年性痴呆的研究。目的:观察电针对麻醉状态下正常和东莨菪碱引起的学习记忆减退模型大鼠海马兴奋性突触后电位(excitatorypostsynapticpotential,EPSP)LTP的作用。设计:随机对照研究。地点和材料:SD大鼠40只,体质量270～310g,单纯随机分成为:对照组、电针组、模型组和模型+电针治疗组,每组10只。干预:引导大鼠海马齿状回颗粒细胞层突触后兴奋性电位群(EPSPs),强直刺激大脑皮质前穿质区引起海马突触LTP反应;用东莨菪碱制备学习记忆障碍模型;电针(疏密波3～5mA,30min)大椎和双侧肾俞穴;观察电针对正常和模型大鼠海马LTP的影响。主要观察指标:强直刺激前和强直刺激诱发后0,60,120minLTP群发电位信号(PS),PS的EPSP潜伏期,PS锋值潜伏期,EPSP的斜率,PS锋值和PS锋面积(PSa)。结果:①电针对强直刺激诱发的海马突触LTP效应的作用强于对照组。与对照组比,EPSP潜伏期明显缩短犤相对于强直刺激前的变化率对照组与电针组分别为(-7.8±2.6)%比(-14.4±7.7)%,差异有显著性意义(t=2.5681,P<0.05)犦;EPSP的斜率增加(t=2.4364,P<0.05);PS锋面积增大(t=2.7508～2.9909,P<0.05),且维持时间长于对照?BACKGROUND:The combination of the researches between long-term potentiation(L TP) of hippocampus and behavioral method of animals has been widely introduced i nto the researches of improving the functions of study and memory,and the treatm ents of Alzheimer's disease including Traditional Chinese Medicine treatments. OBJECTIVE:To observe the impacts of electrical acupuncture(EA) on LTP of excit atory postsynaptic potential(EPSP) in rat hippocampus in normal and scopolamine -induced hypomnesia models under anesthesia conditions. DESIGN:A randomized controlled study. SETTING and MATERIALS;Forty SD rats with a body weight of 270-310 g were rand omly grouped into control group, EA group, model group, and model+EP group with 10 rats each. INTERVENTIONS:EPSPs of granular cell layer at rat hippocampus dentate gyrus we re induced and recorded.LTP reaction of hippocampal synapse was aroused by high frequency stimulating(HFS)anterior perforated substance of cerebral cortex.Hypom nesia model was established by intraperitoneal injection of scopolamine.Dazhui(D U14) and both Shenshu(BL23) acupoints were acupunctured with EA with 3-5 mA of sparse and dense alternation wave for 30 minutes. The impacts of EA on normal an d model hippocampal LTP were observed. MAIN OUTCOME MEASURES:Population spike(PS) ,latency of EPSP,latency of PS peak ,EPSP slope,PS peak value and PS peak area(PSa) of LTP before and at 0,60 and 12 0 minutes after HFS. RESULTS:①The impact of EA on LTP of hippocampal synapse induced by HFS was st ronger than that of control group.Compared with control group,EPSP latency signi ficantly shortenedEffects of electrical acupuncture on long-term potentiation of excit atory postsynaptic potential in rat hippocampus$$$$ Cheng Ma,Li-Ping Yan,Mei-Hong Shen Cheng Ma,Li-Ping Yan,Mei-Hong Shen,Jiangsu Key Laboratory of Acupuncture and Moxibustion,Nanjing University of Traditional Chinese Medicine,Nanjing 210029,J iangsu Province,China Abstract BACKGROUND:The combination of the researches between long-term

关 键 词：电针 大鼠 海马兴奋性突触后电位 海马长时程增强 老年痴呆 学习记忆功能 

分 类 号：R277.791.6[医药卫生—中医学] R245