用重组自交系构建西瓜分子遗传图谱  

Construction of a Molecular Genetic Map for Watermelon Derived from Recombinant Inbred Lines (RILs)

作　　者：张仁兵[1] 易克[2] 许勇[3] 寿森炎[1] 刘立功[3] 张海英[3] 宫国义[3] 

机构地区：[1]浙江大学园艺系,杭州310029 [2]湖南农业大学理学院,长沙410128 [3]国家蔬菜工程技术研究中心,北京100089

出　　处：《分子植物育种》2003年第4期481-489,共9页Molecular Plant Breeding

基　　金：国家自然科学基金项目(30070527);863计划(2001AA241174);北京市自然科学基金重点项目(5991017)资助。

摘　　要：用可溶性固形物含量高、皮薄、感枯萎病的栽培西瓜自交系 (Citrulluslanatusvar lanatus) 9710 3和可溶性固形物含量低、皮厚、抗病的野生西瓜种质 (Citrulluslanatusvar citroides)PI2 96 341杂交所得重组自交系F8的 117个单株为作图群体 ,构建西瓜分子遗传图谱。该图谱包含 87个RAPD标记、 13个ISSR标记和 4个SCAR标记 ,分为 15个连锁群 ,包括 1个最大的含 31个标记的连锁群 (2 77 5cM )、6个大的含 4 - 12个标记的连锁群 (5 1 7cM - 172 2cM )和 8个小的含 2 - 5个标记的连锁群 (7 9cM -46 4cM) ,覆盖基因组的 10 2 7 5cM。两个标记间的平均距离为 11 5 4cM。该图谱为以后获得饱和的分子遗传图谱。A molecular genetic map was constructed for watermelon based on 87 RAPD(random amplified polymorphic DNAs) markers, 13 ISSR(inter simple sequence repeat) markers, and 4 SCAR(sequence characterized amplified region) markers using a RIL population of 117 F 8 progenies derived from the cross 97103(which is a cultivar with higher total soluble solids concentration, thin rind, susceptible to Fusarium wilt disease) × PI296341(which is a wild germplasm with lower total soluble solids concentration, thick rind, resistant to Fusarium wilt disease). The map consists of 15 linkage groups. Among them are a large groups linkage group that contains 31 markers covering a mapping distance of 277 5 cM, six large groups each with 4-12 markers covering a mapping distance of 51 7 to 172 2c M and eight small groups each consisting of 2-5 markers covering a mapping distance of 7 9-46 4 cM. The entire map covers a total distance of 1027 5cM with an average distance of 11 54 cM between two markers. This map is useful for the further development of matured molecular genetic map, analysis of important agricultural characterized quantitative trait loci (QTL) and map based positional cloning for disease resistance gene.

关 键 词：重组自交系 西瓜 分子遗传图谱 农艺性状 RAPD ISSR SCAR 

分 类 号：S651[农业科学—果树学]