豇豆胰蛋白酶抑制剂 (CpTI)抗虫转基因新疆杨的获得  

Transgenic Plantlets of Populus alba var. pyramidalis by Transformation with CpTI Gene

作　　者：诸葛强[1] 王婕琛[1] 陈英[1] 伍宁丰[2] 黄敏仁[1] 范云六[2] 王明庥[1] 

机构地区：[1]南京林业大学林木遗传和基因工程实验室,南京210037 [2]中国农业科学院生物技术研究所,北京100081

出　　处：《分子植物育种》2003年第4期491-496,共6页Molecular Plant Breeding

基　　金：新疆准噶尔生态公司、江苏省自然科学基金(项目编号:BK2001102);江苏省高新技术项目(项目编号:BG2001313)的资肋。

摘　　要：利用根癌农杆菌介导法将广谱抗虫基因豇豆胰蛋白酶抑制剂 (CpTI)基因导入新疆杨 (Populusal bavar pyramidalis) ,经筛选获得了大量转基因植株。对转基因植株经PCR鉴定、PCR Southern和点杂交分子检测结果证实CpTI基因已整合进杨树基因组中。部分转基因植株的饲虫实验表明 。Cowpea trypsin inhibitor(CpTI)gene, an insecticidal gene, was introduced into poplar( Populus alba var. pyramidalis ) by gene transformation mediated by Agrobacterium tumefaciens. Kanamycin resistant(Km r) plantlets were obtained by rigorous selective condition. The presence of CpTI gene in the transgenic plants were confirmed by PCR, PCR Southern and dot Southern blot. The result of insect bioassay showed that some transgenic plants were extremely resistant to the larvae of Apocheimia cinerarius Erschoff, whereas the untransformed control plants was sensitive to them. It appeared that transgenic plantlets had such effects as a decreased leaf consumption by larvae, a lower larvae weight and inhibition to the development of larvae.

关 键 词：豇豆胰蛋白酶抑制剂 抗虫基因 新疆杨 转基因 根癌农杆菌 CPTI基因 

分 类 号：S792.11[农业科学—林木遗传育种]