应用ISSR标记分析灰布支黑豆与晋豆23的F3群体的遗传多样性  

Genetic Diversity of F_3 Progeny Derived from ZDD2315×Jinbean 23 Revealed by ISSR Marker

作　　者：任小俊[1] 马俊奎[1] 章彦[2] 刘学义[1] 

机构地区：[1]山西省农科院经济作物研究所,汾阳032200 [2]海南省农作物分子育种重点实验室,三亚572027

出　　处：《分子植物育种》2003年第5期629-632,共4页Molecular Plant Breeding

基　　金：国家863计划(项目编号:2001AA211101)资助

摘　　要：本研究对晋豆 2 3与大豆孢囊线虫抗源灰布支黑豆的F3代群体共 6 1个单株进行了ISSR分析 ,共检测出 376个基因位点 ,多态性位点 82个 ,多态性位点比例为 2 1 8%。获得 5个共显性位点 ,5个共显性位点的平均基因杂合度为 0 16 4。ISSR引物 (GA) 8C检测到 3种基因型 ,ISSR引物VDV (CT) 7检测到16种基因型。本研究表明该群体内有较丰富的遗传多样性 ,说明灰布支黑豆是大豆孢囊线虫抗病育种的理想抗源。A hundred of ISSR primer were employed to analyze F 3 progeny derived from Jinbean 23 and ZDD2315. A total of 376 loci were detected and 82 loci were polymorphsm, which account for21.8%, Five loci might be co-odominant locus. The average gene heterozygosity of five co dominant loci is 0.164. ISSR primer (GA) 8C generated 3 genotypes and ISSR primer VDA (CT) 7 generated 16 genotypes. The data showed that population had abundant genetic diversity and ZDD2315 was an ideally resistant germplasm to SCN resistant breeding.

关 键 词：ISSR标记 大豆 灰布支黑豆 晋豆23品种 遗传多样性 孢囊线虫 抗病育种 

分 类 号：S565.1[农业科学—作物学]