农杆菌介导法转GMCSF基因玉米研究初报  

Study on Transformation of GM-CSF Gene into Maize Mediated by Agrobacterium Tumefaciens

作　　者：王云翠[1] 邓德祥[1] 姚泉洪[2] 蒋守华[1] 张勇[1] 王益军[1] 范惠琴[2] 

机构地区：[1]扬州大学农学系,扬州225009 [2]上海市农业科学院生物中心,上海201109

出　　处：《分子植物育种》2003年第5期677-681,共5页Molecular Plant Breeding

摘　　要：利用农杆菌介导法将GM CSF转化玉米自交系B73、J7- 3及杂交种B73×A188幼胚 ,获得了B73、B73×A188的潮霉素抗性植株 ,经PCR、PCR Southernblotting检测 ,外源基因均已转入玉米基因组中。Transformation of GM-CSF gene into maize was carried out in this reseach,hygromicin resistant plants of inbred B73 and hybrid B73×A188 were achieved transformed by Agrobacterium Tumefaciens.The results of PCR and PCR-Southern blotting analysis of transformants indicated that exogenes have been inserted into maize genome.

关 键 词：GM-CSF基因 玉米 转基因 农杆菌介导 潮霉素 粒细胞巨噬细胞集落刺激因子 细胞因子多肽药物 基因工程药物 

分 类 号：S513[农业科学—作物学]