SLE患者血清中SARS-CoV抗体阳性原因分析  

ANALYSIS OF FALSE-POSITIVE ASSOCIATED WITH ANTIBODY TESTS FOR SARS-COV IN SLE PATIENTS

作　　者：汪运山[1] 申红[1] 孙善会[1] 姜莉华[1] 刘洋[1] 朱之炜[1] 肖东杰[1] 黄萍[1] 杨博[1] 杜希燕[1] 张源潮[2] 

机构地区：[1]山东大学临床医学院济南市中心医院中心实验室,济南250013 [2]山东省立医院风湿免疫科,济南250021

出　　处：《实验生物学报》2003年第4期314-317,共4页Acta Biologiae Experimentalis Sinica

摘　　要：为了探讨严重急性呼吸综合征(SARS)冠状病毒(SARS-CoV)抗体测定在系统性红斑狼疮(SLE)患者中的假阳性问题,应用酶联免疫吸附试验(ELISA)和荧光定量RT-PCR技术检测了66例正常对照和31例SLE患者血清中SARS-CoV抗体的阳性率。结果,66例正常对照中,IgM抗体均阴性,IgG抗体的阳性率为3.0%(2/66);31例SLE患者中,IgM抗体和IgG抗体阳性率分别为29%(9/31)和58.1%(18/31),IgG抗体和IgM抗体同时阳性为22.6%(7/31)。经RTPCR检测,上述阳性病例均为阴性。结论:用非纯化抗原制备的ELISA试剂盒测定SLE患者的SARS-CoV抗体,可能出现假阳性,两种抗体同时测定可降低诊断的假阳性率,提高诊断的特异性。在SLE患者中出现假阳性的原因可能与包被的抗原有关。To discuss the fasle-positive of serological diagnostic testing for coronavirus antibody in patients with systemic lupus erythematosus(SLE), 66 normal individual and 31 SLE with non-SARS patients were detected for SARS-associated coronavirus (SARS-CoV) antibody and RNA by enzyme-linked immunosorbent assays(ELISA) and reverse transcriptase-polymerase chain reaction(RT-PCR). The result showed 2/66 cases(3. 0%) were positive of SARS-CoV-IgG antibody and 66 cases were negative of SARS-CoV-IgM antibody in the 66 cases healthy controls; in 31 cases with SLE, positive rates of SARS-CoV-IgG and IgM antibody were 58. 1 % ( 18/31 ) and 29% (9/31), respectively, in which 7 cases(22. 6% ) were positive of both SARS-CoV-IgG and IgM antibody. All samples of positive SARS-CoV-IgG and IgM antibody were negative by RT-FCR. The ELISA kit coated by non-purification antigen may induce the false-positive of SARS-CoV antibody in patients with SLE. This result suggested that the specificity of ELISA tests for SARS was excellent and has low fasle-positive rates when using SARS-CoV-IgG and IgM antibody tests .A possible cause of false-positive of SARS-CoV-IgG and IgM antibody in SLE patients is coated antigens with SARS-CoV and Vero-E6 cells in ELISA methods.

关 键 词：严重急性呼吸综合征 SARS 抗体 系统性红斑狼疮 特异性 SLE患者 血清 冠状病毒 

分 类 号：R593.241[医药卫生—内科学]