当归补血汤对实验性高眼压兔视网膜神经节细胞保护作用的研究  

The protection of dangguibuxuetang(当归补血汤)on the retinal ganglion cells of rabbits with experimental intraocular hypertension

作　　者：李德姣[1] 蒋伯龄[2] 朱元莉[3] 

机构地区：[1]中日友好医院眼科,北京100029 [2]中国中医研究院广安门医院眼科 [3]北京大学第三医院病理科

出　　处：《中国中医眼科杂志》2004年第2期73-76,共4页China Journal of Chinese Ophthalmology

摘　　要：目的　观察当归补血汤对实验性高眼压兔眼压得到一定程度控制情况下视网膜神经节细胞(retinalganglioncells ,RGCs)保护作用。方法　用 0 4 %的复方卡波姆前房注射造成兔实验性高眼压模型。分成 4组 ,正常组、Timolol组、Timolol加中药组、高眼压未治疗组。观察 4、 6、 8周后处死 ,制作视网膜病理切片 ,图像分析仪计数视网膜铺片RGCs数及视网膜神经纤维层 (retinalnervefiberlayer ,RNFL)厚度。结果　联合给药组RGCs数 75 7 89± 134 74 (个 /mm2 ) ,明显高于高眼压未治疗组 4 33 2 9± 119 6 6 (个/mm2 ,P <0 0 1)。联合给药组RNFL厚度 31 2 8± 2 0 1(μm) ,与正常组之间差异无显着性 ,但明显高于高眼压对照组 (P <0 0 1)和噻吗心安组 (P <0 0 5 )。结论　在眼压得到一定程度的控制下 ,当归补血汤可以提高RGCs存活率 ,减轻RNFL损害 。OBJECTIVE To investigate protective effect of dangguibuxuetang(当归补血汤) on retinal ganglion cells in rabbits with experimental ocular hypertension when intraocular pressure were controlled to some degree METHODS Experimental ocular hypertension was produced by injecting 0 4% carbomer into anterior chambers The rabbits were divided into 4 groups: normal group、timolol group(T), timolol plus Dangguibuxuetang group(TC), and untreated control group(EP) At 4,6,and 8 weeks the rabbits were killed; retinas were obtained for pathological analysis The image analysis system was used for measuring the thick of nerve fiber layer of retina and RGCs density of flat mount preparation RESULTS RGCs of TC group 757 89±134 74(cells/mm 2), increased remarkably than EP group 433 29±119 66(cells/mm 2)( P <0 01);Thick of RNFL:TC group 31 28±2 01(μm),as thick as normal group, had significant difference with EP group( P <0 01)and T group( P <0 05) CONCLUSION When IOP controlled to some degree, dangguibuxuetang can protect RGCs by increasing the survival of RGCs,diminishing RNFL damage

关 键 词：当归补血汤 视网膜神经节细胞 高眼压 

分 类 号：R285.5[医药卫生—中药学]