反相高效液相色谱法测定己烯雌酚乳膏的含量  

Determination of content of diethylstilbestrol in creams by HPLC

作　　者：袁晔[1] 张蓓[2] 陈建[1] 郭玉萍[2] 

机构地区：[1]天津市药品检验所,天津300070 [2]天津市天和医院,天津300050

出　　处：《天津药学》2004年第2期14-16,共3页Tianjin Pharmacy

摘　　要：目的 :建立测定 0 .0 1%己烯雌酚乳膏含量的分析方法。方法 :采用反相高效液相色谱法 ,Kromasil ODS色谱柱 ( 2 5 0 mm× 4 .6 mm,5 μm) ,以甲醇 -水 ( 80∶ 2 0 )为流动相 ,流速 0 .6 ml/min,检测波长 2 5 4 nm,柱温 :室温 ,进样量 2 0μl。结果 :己烯雌酚的浓度在 5 .0 0～ 15 .0 0 μg/ml范围内线性关系良好 ,相关系数 r=0 .9997;乳膏中己烯雌酚的平均回收率为 98.82 % ,RSD=0 .6 9% ( n =9) ;重现性良好 ( RSD=0 .5 1% )。结论 :该法操作简便、快速、准确 。Object To determine the content of diethylstilbestrol in creams. Methods A HPLC method was used. The mobile phase consisted of methanol water(80:20), at a flow rate of 0.6 ml/min 1 . The detection wavelength was 254nm. Results The linear calibration curves were obtained over the range of 5～15 μg/ml -1 (r=0.9997). The average recovery of diethylstilbestrol was 98.82% (RSD 0.69%) in creams. The precision of the method (RSD 0.51%)was good. Conclusion The method was simple, rapid and accurate. It was suitable for detecting the content of diethylstilbestrol in creams,

关 键 词：己烯雌酚乳膏 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]