中药益气化瘀方对体外培养的大鼠颈椎间盘软骨细胞生物学功能的影响  

THE INFLUENCE OF TCD QI-SUPPLEMENTING AND BLOOD-STASIS-REMOVING RECIPE ON THE BIOLOGICAL FUNCTION OF IN-VITRO-CULTIVATED RAT CERVICAL INTERVERTEBRAL DISK CHONDROCYTES

作　　者：许理忠[1] 王拥军[2] 周重建 刘梅 侯宝兴[1] 施杞[2] 

机构地区：[1]上海中医药大学附属龙华医院,200032 [2]上海中医药大学脊柱病研究所

出　　处：《中医正骨》2004年第5期3-6,共4页The Journal of Traditional Chinese Orthopedics and Traumatology

基　　金：上海市科技启明星项目 (编号 :0 1QB14 0 39)

摘　　要：为研究中药益气化瘀方对体外培养的大鼠颈椎间盘软骨细胞生物学功能的影响。取健康 10周龄SD大鼠 70只 ,体重 2 0 0± 5 0g ,雌雄各半 ,随机分为空白组 (E)、芬必得组 (D)、中药低剂量组 (C)、中药中剂量组 (B)、中药高剂量组 (A) ,每组 14只。定时、定量胃饲 ,4天后分别处死取血清 ,相同条件下制备益气化瘀方含药血清和对照血清 ,取体外培养的大鼠颈椎间盘软骨细胞进行实验研究 ,采用MTT法观察软骨细胞生长、增殖 ;流式细胞仪检测细胞凋亡率。结果显示不同中药浓度对培养的软骨细胞生长、增殖均有促进作用 ,以 10 %血清浓度高剂量含药血清组促进软骨细胞增殖的作用最为明显 ,与其他各组相比 ,有非常显著的差异 (P <0 .0 1)。细胞周期分析 ,中药组能促进细胞DNA合成 (S期 ) ,与对照组相比 ,有非常显著的差异 (P <0 .0 1)。表明益气化瘀方具有促进大鼠颈椎间盘软骨细胞生长、增殖及促进细胞DNA合成的作用。The objective of the paper is to study the effect of TCD Qi-supplementing and blood-stasis-removing prescription (QBP) on the biological function of in-vitro cultivated rat cervical intervertebral disk chondrocytes. Seventy healthy SD rats with body weight: 200±50g and aged 10 months (males and females half each) were randomly divided into five groups (n=14 each): Group I as the blank treated by normal saline, Group II as the control treated by Fenbid, Group III treated by low-dose QBP, Group IV treated by middle-dose QBP, Group V treated by high-dose QBP; the administration was both quantitative and regular and lasted four days; four days later, the serums of each group were taken and, under the same conditions, made into the QBP-containing and the control serum, in which the rat cervical intervertebral disk chondrocytes were cultivated in vitro; the growth and proliferation of the chondrocytes were observed with MTT method; and the cellular withering rate was detected with a flow cytometer. The results showed that all of three doses of QBP functioned to promote the growth and proliferation of the chondrocytes cultured in vitro; the high dose (that is, 10 %-QBP-serum) had the greatest function in the three doses with a significant difference from other groups (P < 0.01); the cell cycle analysis showed that QBP could promote the cell DNA synthesis with a very significant difference from the control group (P < 0.01), suggesting that QBP functions to promote the growth, proliferation and DNA synthesis of rat cervical intervertebral disk chondrocytes.

关 键 词：软骨细胞 益气化瘀方 体外培养 大鼠 

分 类 号：R285[医药卫生—中药学]