紫外吸光光度法测定蔬菜鲜样中硝酸盐氮  

UV-SPECTROPHOTOMETRIC DETERMINATION OF NITRATES IN FRESH VEGETABLES

作　　者：冷家峰[1] 刘仙娜 王泽俊[1] 

机构地区：[1]济南市环境保护科学研究所,济南250014

出　　处：《理化检验（化学分册）》2004年第5期288-289,共2页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：采用紫外吸光光度法测定蔬菜鲜样中硝酸盐含量,该法适用于不同硝酸盐含量的蔬菜样品测定,与传统的酚二磺酸法相比,该法精密度和准确度高、操作简便,具有较大的推广价值。Based on its characteristic absorption at 220nm, nitrates in fresh vegetables were determined by UV-spectrophotometry. Nitrates in the sample were extracted into an ammoniacal buffer at pH 9.6～9.7. By adding some activated charcoal to the buffer solution the pigments were removed simultaneously. The protein was removed by adding potassium ferrocyanide and zinc sulfate as precipitant. The interference of nitrite was eliminated by addition of sulfamic acid before the absorption measurement. This method has the special features of high accuracy and precision, and more convenient in operation. In comparison with the conventional phenol disulfonic acid method, no significant difference was found.

关 键 词：紫外吸光光度法 蔬菜 硝酸盐 氮 酚二磺酸法 色素 活性碳 

分 类 号：X836[环境科学与工程—环境工程]