文心兰组织培养的研究  

Studies on Tissue Culture of Oncidium

作　　者：崔广荣[1] 刘云兵[1] 张俊长[1] 谷业理[1] 

机构地区：[1]安徽技术师范学院农学系,凤阳233100

出　　处：《园艺学报》2004年第2期253-255,共3页Acta Horticulturae Sinica

基　　金：安徽技术师范学院自然科学基金项目(2003Y207)

摘　　要：以文心兰不同发育时期的花蕾为外植体,研究了影响丛芽诱导、增殖、生根壮苗的主要因素。结果表明,处于原芽期的花蕾是诱导丛芽的较好材料;适合花原芽诱导丛芽的培养基是MS+6-BA 4.0 mg/L+NAA 0.5 mg/L+3％蔗糖,适合丛芽增殖成苗的培养基是MS+6-BA 2.0-4.0 mg/L+NAA 0.5 mg/L+3％蔗糖,适合生根壮苗的培养基为1/2 MS+IBA 0.5 mg/L+2％蔗糖。The main factors of crowd shoot induction, propagation and rooting of Oncidium were studied by using different growth period of bud. The results showed that the primary bud is better explants than others. The different kinds and different concentration of hormones greatly affected crowd shoot induction, propagation and rooting of Oncidium. The suitable culture medium for crowd shoot induction is MS + 6-BA 4. 0 mg/L + NAA 0. 5 mg/L + 3% sucrose. The best culture medium for shoot propagation is MS + 6-BA 2.0-4. 0 mg/L + NAA 0. 5 mg/L + 3% sucrose. The proper culture medium for rooting is 1/2 MS + IBA 0. 5 mg/ L +2% sucrose.

关 键 词：文心兰 组织培养 外植体 培养基 细胞分裂素 生长素 

分 类 号：S682.31[农业科学—观赏园艺]