人外周血网织红细胞微核作为辐射生物剂量仪的初步研究  

An Primary Study on Micronucleated Reticulocytes from Human Peripheral Blood as a Biodosimeter for Radiation

作　　者：孙立平[1] 李德志[2] 刘志谋[2] 杨录军[1] 刘晋祎[1] 曹佳[1] 

机构地区：[1]第三军医大学卫生毒理学教研室,重庆400038 [2]第三军医大学附属新桥医院肿瘤治疗中心,重庆400037

出　　处：《癌变．畸变．突变》2004年第3期163-166,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家杰出青年基金资助项目(No.30125037) ;军队杰出青年基金资助项目(No.01J014)

摘　　要：背景与目的 :探讨人外周血网织红细胞微核率是否可以替代双核淋巴细胞微核率作为辐射生物剂量仪。材料与方法 :以接受放疗的鼻咽癌(nasopharyngealcancer,NPC)患者为受辐照模型 ,以双核淋巴细胞微核实验为比对标准 ,应用已建立的基于单激光流式细胞仪的人外周血网织红细胞微核自动化检测体系 ,检测NPC患者接受不同累积放疗剂量后的外周血网织红细胞微核率。结果 :NPC患者接受放疗后 ,外周血网织红细胞微核率迅速显著增高 ,呈现良好的剂量_反应关系 ;外周血网织红细胞微核率对辐射的反应模式与双核淋巴细胞微核率对辐射的反应模式一样 ,所得曲线方程均符合 ^Y=A +BX -CX2+DX3模式。结论BACKGROUND&ATM: To study whether the frequency of micronucleated reticulocytes(fMN-Trf-Ret)from human peripheral blood can be used as a biodosimetry to estimate damages caused by radiation. MATERIAL AND METHODS: Nasopharyngeal cancer(NPC)patients were used as models for victims of radiation and their fMN-Ret from peripheral blood were detected after accepting different cumulative doses of radiotherapy using the automatic scoring system for micronucleus test(MNT)based on a single-laser flow cytometer which was established before;cytokinesis block micronucleus test(CB-MNT)was performed to set a standard for comparison. RESULTS: Both the fMN-Ret and the frequency of micronucleated binucleated lymphocytes(fMN-BNLs)of NPC patients were elevated rapidly and sharply after accepting radiotherapy,showing good dose-response relationships;the fMN-Trf-Ret reacted to radiation as the fMN-BNL did. CONCLUSION: The fMN-Trf-Ret from human peripheral blood is a good substitute to the fMN-BNL as a biodosimetry to estimate damages caused by radiation.

关 键 词：微核实验 流式细胞仪 自动化检测 辐射生物剂量仪 人外周血 

分 类 号：R944.6[医药卫生—药剂学]