咖啡酸对神经毒素MPP^+诱发大鼠小脑颗粒细胞凋亡的保护作用  

Caffeic acid (CA) protects cerebellar granule neurons (CGNs) from apoptosis induced by neurotoxin 1-methyl-4-phenylpyridnium (MPP^+)

作　　者：田雪菲[1] 蒲小平[1] 

机构地区：[1]北京大学药学院分子与细胞药理学系,北京100083

出　　处：《北京大学学报（医学版）》2004年第1期27-30,共4页Journal of Peking University:Health Sciences

基　　金：国家重点基础研究发展规划项目基金 (G19980 5 112 4)资助~~

摘　　要：目的 :探讨咖啡酸 (CA)是否对 1 甲基 4 苯基吡啶离子 (1 methyl 4 phenylpyridiniumion ,MPP+ )诱导的大鼠小脑颗粒细胞凋亡具有保护作用。方法 :用咖啡酸孵育大鼠小脑颗粒细胞 ,经MPP+ 处理后 ,用MTT法测定细胞存活率 ,荧光法检测caspase 3的活性。 结果 :咖啡酸对MPP+ 致小脑颗粒细胞存活率降低和caspase 3活性增强具有很好的抑制作用 ,并呈现稳定的量效和时效关系。结论 :咖啡酸对MPP+Objective: To assess the effects of caffeic acid (CA) on MPP + induced cerebellar granule neurons (CGNs) apoptosis. Methods: CGNs were pretreated with caffeic acid at 55, 110 and 220 μmol/L for 6 h, then treated with 100 μmol/L MPP + for 24 h (concentration effect relationship). In addition CGNs were pretreated with caffeic acid at 110 μmol/L for 0 h, 6 h, 12 h, and 24 h, respectively, then treated with 100 μmol/L MPP + for 24 h (time response relationship). Besides, after treatment with MPP + for 24 h, CGNs were incubated with caffeic acid at 55, 110 and 220 μmol/L,respectively. Cell viability was determined by 3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyltetrazolium bromide (MTT) assay and caspase 3 activity was assayed by caspase 3 fluorometric assay kit. Results: MTT assay revealed that caffeic acid significantly inhibited cell viability decrease induced by MPP +, and caspase 3 fluorometric assay showed that caffeic acid efficiently suppressed caspase 3 activation in CGNs induced by MPP +. Conclusion: Caffeic acid (CA) can significantly protect CGNs from apoptosis induced by MPP + and may provide a useful therapeutic strategy for the treatment of Parkinson's disease.

关 键 词：咖啡酸 神经毒素 半胱氨酸天冬氨酸蛋白酶 细胞凋亡 大鼠 帕金森病 小脑颗粒细胞 

分 类 号：R966[医药卫生—药理学] R742.5[医药卫生—药学]