X射线照射对视神经间接损伤后再生的作用  

Role of X-ray irradiation on regeneration of rabbit optic nerve after indirect injury

作　　者：李德忠[1] 丁继兵[1] 吴建国[2] 

机构地区：[1]广州军区武汉总医院医学实验科电镜室,湖北武汉430070 [2]广州军区武汉总医院眼科,湖北武汉430070

出　　处：《电子显微学报》2004年第2期139-143,共5页Journal of Chinese Electron Microscopy Society

摘　　要：为了探讨X射线对视神经间接损伤后轴突再生的促进作用和对胶质细胞增生的抑制作用,用光镜和电镜对用特制装置致伤的,在不同时间、用不同X射线剂量照射的视神经进行了观察。结果显示:在损伤后1、2、3、4周35GyX射线照射组动物的视神经再生率分别为95%、74%、27%和33%,特别是在1、2周组电镜下可见结构良好的再生神经轴突。在45Gy和55Gy组,随着X射线量增大、照射时间后移,视神经的再生减少,直至消失。另外,35、45、55GyX射线量对视神经间接损伤引起的胶质细胞增生均有明显的抑制作用。效果以35Gy1周组最好,随X射线量增大和照射时间后移其抑制作用减弱。结论:视神经间接损伤后,早期适量的X射线局部照射能有效地抑制胶质细胞的反应性增生,能明显地促进神经轴突的结构再生。In order to explore the promotive role of X-ray on axonal regeneration of optic nerve and its inhibitive action on glial cell proliferation after indirect injury caused by using special injurious equipment,we observed the injuried optic nerves radiated by different X-ray doses(35,45,55 Gy) and in different stages after injary under light and electron microscope.The results showed that numerous axons with well developed neurofube and neuro‐filaments could be seen in the tissues of the first and second week's groups with 35Gy X-ray irradiation,and some axons in the first week after irradiation with 45Gy.There was a less axonal regeneration in the third week 35Gy and the second week 45Gy irradiation groups.There was no axonal regeneration in the forth week of 35Gy,the third and the forth week of 45Gy week and all of the 55Gy irradiation groups.The glial cell proliferation in the injured optic nerve was obviously inhibited by 35、45 and 55Gy irradiation doses but the degree of inhibition reduced gradually as the X-ray-does increased.It is suggested that early proper X-ray irradiation on the injured optic nerve can effectively inhibit the proliferation of astrocytes and obviously promote the axonal regeneration of optic nerve.

关 键 词：X射线照射 神经再生 视神经 胶质细胞 超微结构 反应性增生 

分 类 号：R818[医药卫生—放射医学]