硝普钠对脊髓源性神经干细胞增殖抑制及诱导分化作用  

Effects of sodium nitroprusside on proliferation and differentiation of spinal neural stem cells

作　　者：刘艳武[1] 罗卓荆[1] 曹延林[1] 叶正旭[1] 

机构地区：[1]第四军医大学西京医院全军骨科研究所,陕西西安710033

出　　处：《第四军医大学学报》2004年第9期791-794,共4页Journal of the Fourth Military Medical University

基　　金：国家"8 63"课题 (2 0 0 2AA2 1 61 0 1 )

摘　　要：目的 :探讨硝普钠 (SNP)对脊髓源性神经干细胞增殖的抑制作用及诱导其向神经细胞分化的可能性 .方法 :脊髓源性的神经干细胞在无血清培养液中培养 ,加入不同浓度的硝普钠溶液 ,采用细胞培养技术结合间接免疫细胞化学法 ,观察神经干细胞的数量、形态变化 .结果 :硝普钠作用后活细胞的数量较对照组明显减少 ,硝普钠可以抑制脊髓源性神经干细胞的增殖 [对照组与实验组的细胞数分别为 (5 .1±1 .3)× 1 0 5/mL ,(3.3± 0 .8)× 1 0 5/mL ,(2 .2± 0 .6 )× 1 0 5/mL和(1 .4± 0 .3)× 1 0 5/mL ,其中组 3与组 4间P <0 .0 5 ,其余各组间P <0 .0 1 ].相差显微镜下大部分神经干细胞伸出多个细长突起 ,且MAP2免疫荧光染色增强 ;部分为有多个短小突起、GFAP阳性表达 ,部分细胞为少数粗大突起 ,MAG染色阳性 .神经元数与 3种细胞总数之比 ,4组神经元的比例分别为 (30 .2± 3.8) % ,(5 0 .9± 2 .8) % ,(5 8.6± 3.3) %和 (6 6 .7± 4 .1 ) % ,硝普钠浓度越高 ,神经元比例越大 (P <0 .0 1 ) .结论 :硝普钠可以抑制脊髓源性神经干细胞的增殖 .硝普钠还可诱导脊髓源性神经干细胞向神经元的分化 ,而抑制其向星型胶质细胞分化 .浓度越大 。AIM: To investigate effects of sodium nitroprusside (SNP) on the proliferation and the differentiation of spinal neural stem cells into neural cells in vitro . METHODS: Spinal neural stem cells were cultured on the media without serum and with different concentration of SNP. The quantitative and morphological changes of the cells and the expression of MAP2, GFAP and MAG were observed and photomicrographed by phase contrast microscope. RESULTS: The number of living cells in the experiment groups was fewer than that in the control group [ (3.3± 0.8) ×10 5/mL, (2.2±0.6)×10 5/mL and (1.4±0.3)×10 5/mL, vs (5.1±1.3)×10 5/mL, P <0.05]. The majority of the spinal neural stem cells protruded with several long processes and expressed high level of MAP2, while the minority of the spinal neural stem cells protruded with several short processes which were GFAP positive. The remaining small part protruded with several crassitude processes which were MAG positive. The ratio of neurons was different in control and experimental groups [(30.2±3.8)% vs (50.9±2.8)%, (58.6±3.3)% and (66.7±4.1)%, P <0.01]. CONCLUSION: Sodium nitroprusside can inhibit the proliferation and promotes the differentiation of spinal neural stem cells into neuronal but not glial cells in vitro in a concentration dependent manner.

关 键 词：神经干细胞 神经细胞 硝普钠 增殖抑制 分化 脊髓 

分 类 号：R329.33[医药卫生—人体解剖和组织胚胎学]