核心蛋白多糖及其mRNA在正常皮肤和增生性瘢痕组织中的表达  

The content of decorin and its mRNA expression in normal human skin and hyperplastic scars

作　　者：张志[1] 刘琰[1] 章雄[1] 许伟石[1] 

机构地区：[1]上海第二医科大学瑞金医院烧伤科,200025

出　　处：《中华烧伤杂志》2004年第2期76-78,共3页Chinese Journal of Burns

摘　　要：目的　检测不同时期增生性瘢痕组织中核心蛋白多糖的含量及其mRNA的表达水平 ,探讨核心蛋白多糖含量的变化与其合成的关系。　方法　取 10例手术剩余的正常皮肤、包皮和 2 2例手术切除的瘢痕组织作标本 ,采用免疫组织化学和蛋白免疫印迹法检测核心蛋白多糖在标本组织中的含量及其分布 ,用原位杂交技术检测其mRNA表达水平。　结果　正常皮肤的真皮中核心蛋白多糖含量丰富 ,mRNA呈低表达。增生性瘢痕组织中核心蛋白多糖含量在 6个月内 (184 0 3± 7193)极少 ,7～ 12个月 (4 2 937± 96 6 2 )逐渐增加 ,13～ 36个月 (86 2 31± 16 2 85 )持续增加 ,36个月以后 (13094 3± 174 5 8)其含量与正常皮肤比较 ,差异无显著性意义 (P >0.0 5);其mRNA水平在 6个月内低于正常 ,7～ 12个月呈高表达 ,13～ 36个月呈持续高表达 ,36个月后逐渐下降至正常皮肤表达水平。　结论　增生性瘢痕组织中核心蛋白多糖减少的主要原因是其合成减少 ,核心蛋白多糖的增加与瘢痕组织稳定的时间相一致 ,提示核心蛋白多糖的延迟出现可能与增生性瘢痕的形成有关。Objective To investigate the content of decorin and its mRNA expression in normal human skin and hyperplastic scars at different stages, so as to explore the relationship between the change of decorin and its synthesis. Methods Scar tissue samples from 22 patients undergoing scar excision and 10 specimens of normal skin or prepuce were obtained. The content and distribution of decorin in the tissue samples were determined with immunohistochemistry and Western blot, and the expression of decorin mRNA was detected by in situ hybridization. Results The content of decorin was rich in the normal skin dermis with lower expression of the mRNA. In contrast, the decorin content was scarce in hyperplastic scars (HS) within 6 months, but increased gradually beginning from 7 to 12 months, and increased continuously for 13 to 36 months. There was no difference between the decorin content in normal skin and that in HS after 36 months (P >0.05). Furthermore, the mRNA expression level in HS tissue was lower than that in normal skin within 6 months, but increased from 7 to 12 months. The mRNA expression continuously increased during 13 to 36 months and then returned to the level similar to that in normal skin thereafter. Conclusion The decrease of decorin in hyperplastic scar was resulted primarily from reduced synthesis. The increase in decorin level coincided with the time of scar tissue stabilization,which implied that the delayed appearance was correlated with the formation of HS.

关 键 词：核心蛋白多糖 MRNA 皮肤组织 增生性瘢痕 免疫组织化学 蛋白免疫印迹法 

分 类 号：R622[医药卫生—整形外科]