腱糖蛋白C在瘢痕疙瘩和增生性瘢痕中的表达  

Study on the expression of tenascin-C in keloid and hyperplastic scar

作　　者：贺肖洁[1] 韩春茂[1] 

机构地区：[1]浙江大学医学院附属第二医院烧伤科,杭州310009

出　　处：《中华烧伤杂志》2004年第2期79-81,共3页Chinese Journal of Burns

摘　　要：目的　观察腱糖蛋白C(Tn C)在瘢痕疙瘩和增生性瘢痕中的表达规律。　 方法　取瘢痕患者的瘢痕疙瘩和 6～ 10个月增生性瘢痕组织各 10例 ,并取部分患者距瘢痕边缘 1cm的正常皮肤 ;另取手术剩余的正常成人皮肤组织 5例。采用免疫组织化学方法 ,检测Tn C在瘢痕疙瘩、增生性瘢痕和正常成人皮肤中的表达。　结果　正常成人皮肤中Tn C表达稀少 ,局限在真皮 表皮交界的乳头真皮 ,部分靠近基底膜血管和皮肤附件。Tn C在瘢痕疙瘩和增生性瘢痕的真皮瘢痕组织、皮肤附件中呈弥散分布 ,表达显著增强 (P <0.0 1),以瘢痕疙瘩的增强尤为明显。瘢痕疙瘩旁正常皮肤附件的Tn C较正常皮肤表达增强 ,在增生性瘢痕旁的正常皮肤中未见到Tn C的高表达。　结论　Tn C在瘢痕疙瘩和 6～ 10个月增生性瘢痕中呈异常高表达 ,值得进一步研究。Objective To investigate the expression of tenascin-C (Tn-C) in keloid and hyperplastic scar (HS). Methods Tissue samples were harvested from 10 patients with keloid and 10 with HS (6-10 months) and from the skin of 5 adult healthy volunteers. The expression of Tn-C in these samples was determined with immunohistochemistry method. Results There was scarce expression of Tn-C in the skin tissue in adult healthy volunteers, and it was only present in the dermal papillae at the dermis epidermis conjunctions and partly in the blood vessels and skin appendages adjacent to the basement membrane. There was enhanced expression of Tn-C in the dermal scar tissue and skin appendages in both keloid and HS, especially in keloid, which exhibited a diffused pattern in the tissue. When compared with that in normal skin, the Tn-C expression in the normal skin adjacent to the keloid was enhanced markedly, but not in the normal skin near HS tissue. Conclusion There was increased Tn-C expression in keloid and HS (6-10 months).

关 键 词：腱糖蛋白C 瘢痕疙瘩 增生性瘢痕 免疫组织化学 细胞外基质 成纤维细胞 

分 类 号：R622[医药卫生—整形外科]