新型增生性瘢痕裸鼠动物模型的建立  

Establishment of an animal model of human hyperplastic scar in nude mice

作　　者：杨东运[1] 李世荣[1] 李钢[1] 刘剑毅[1] 王珍祥[1] 毋巨龙[1] 陈艳清[1] 

机构地区：[1]第三军医大学西南医院整形科,重庆400038

出　　处：《中华烧伤杂志》2004年第2期82-84,共3页Chinese Journal of Burns

基　　金：国家自然科学基金资助项目 ( 3 0 0 0 0 175 )

摘　　要：目的　建立一种稳定的增生性瘢痕 (HS)动物模型 ,为进一步研究HS的发病机制提供帮助。　方法　在 10 0只裸鼠背部分别作 2 .0cm× 1.5cm全层皮肤缺损创面 ,移植人全厚皮肤 ;皮片存活后用加热的铜棒造成深Ⅱ度烫伤 ,观察创面愈合后的瘢痕增生情况。　结果　皮片完全存活的 86只裸鼠中 ,6 7只有明显、持续的瘢痕增生 ,占 78%。增生瘢痕的外观和组织学特点与人体增生性瘢痕相似。瘢痕增生的平均厚度为 0 .34cm,最厚 0 .6 0cm,增生时间为 6 3～ 2 17d,平均 12 8d。结论　该模型瘢痕增生明显、增生持续时间长 ,可用于观察创面愈合至瘢痕形成的全过程 ,是目前较为稳定的增生性瘢痕动物模型。Objective To establish an ideal model of human hyperplastic scar (HS) in nude mice, so as to provide us a new model to carry out further studies on the mechanism of HS development. MethodsFull skin defect sized 2.0 cm×1.5 cm was created on the back of 100 nude mice. The defect was thereafter covered with full thickness human skin. After the grafted skin survived, the nude mice were subjected to deep partial thickness burn of the grafted skin with heated copper rod. The development of the hyperplasia of the scar after wound healing was observed histologically and grossly. Results Grafted full-thickness human skin took and survived well in 86 out of 100 nude mice. There was obvious and continuous hyperplasia of scar in 67 mice (78%). The external appearance and histological features of the HS appeared similar to those in human HS. The average thickness of the scar was 0.34 cm, with the thickest part measuring 0.6 cm. In addition, the time of hyperplastic change lasted for 63-217 days in average of 128 days. Conclusion Obvious and continuous scar hyperplasia could be found in this model, and the whole process beginning from wound healing to the formation of HS could be easily observed. The model was therefore suitable and ideal for the study of HS.

关 键 词：增生性瘢痕 动物模型 发病机制 皮肤缺损 整形外科 烧伤 

分 类 号：R622[医药卫生—整形外科]