妥泰对急性分离的大鼠海马神经元钠电流的影响  

Effect of TPM on Sodium Currents in Acutely Isolated Rat Hippocampal Neurons

作　　者：朱遂强[1] 笱玉兰 李卫华[1] 唐明[2] 杨美兰[1] 阮旭中[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院神经内科,武汉430030 [2]华中科技大学同济医学院基础医学院生理学教研室,武汉430030

出　　处：《华中科技大学学报（医学版）》2004年第2期146-149,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的　在离子通道水平探讨妥泰 (Topiramax ,TPM)对大鼠海马神经元细胞的钠通道的作用。 方法　采用 7～ 10d的大鼠 ,以链霉素蛋白酶E加吸管吹打法制备海马神经元 ;利用全细胞膜片钳技术 ,观察TPM对急性分离大鼠海马神经元电压依赖性钠通道的作用。结果　TPM使这种河豚毒素 (Tetrodotoxin ,TTX)敏感的内向电流幅值减小 (n =2 7) ,这种作用具有浓度依赖性 ,它还可以使钠电流的稳态失活曲线负向漂移 (n =5 )。Objective To explore the mechanism of topiramax (TPM) on sodium channels of hippocampal neurons in rats. Methods Hippocampal neurons of rats (7 10 days) were freshly dissociated and effects of TPM on voltage dependent sodium current were observed by a whole cell voltage clamp technique.Results These tetrodotoxin sensitive inward currents were reduced by TPM in a concentration dependent manner. Dose response analysis of these effects revealed an IC 50 of 47.6 μmol/L ( n =27); Also TPM made the stead state inactivation curve of this current shift toward more negative values ( n =5).Conclusion TPM have the same effect on sodium current as traditional anticonvulsant drugs.

关 键 词：海马 神经元 膜片钳技术 钠电流 妥泰 抗痫药物 

分 类 号：R971.6[医药卫生—药品]