骨髓基质干细胞分化为胰岛样细胞的形态学观察  

Morphological changes of islet-like cells differentiated from marrow mesenchymal stem cells

作　　者：陈立波[1] 姜小兵[1] 王春友[1] 杨炼[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院普外科,武汉430022

出　　处：《中华实验外科杂志》2004年第2期184-185,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目 (30 1 70 91 1 ) ;湖北省科技攻关计划 (2 0 0 2AA30 1C2 6)

摘　　要：目的　探讨体外自体骨髓基质干细胞 (MSC)定向诱导分化为胰岛样细胞的可能性。方法　对Wistar大鼠MSC进行体外培养 ,通过不同的条件诱导MSC向胰岛样细胞分化 :LN组 ,10mmol/L尼克酰胺 + 1mmol/Lβ 巯基乙醇的低糖Dulbecco最低必须培养基 [L DMEM ,含 2 0 %胎牛血清 (FSC) ]预先诱导 2 4h ,10mmol/L尼克酰胺 + 1mmol/Lβ 巯基乙醇的无血清高糖Dulbecco最低必须培养基 (H DMEM )诱导 10h。HN组 ,含 2 0mmol/L尼克酰胺的L DMEM (2 0 %FSC)预先诱导 2 4h ,2 0mmol/L尼克酰胺的无血清H DMEM诱导 10h。通过倒置显微镜观察诱导细胞的形态学变化。结果　LN、HN组细胞均可见部分骨髓基质干细胞分化、增殖 ,形成胰岛样细胞团 ,部分细胞向神经元样细胞分化 ;对照组细胞未见明显分化。结论　体外诱导骨髓基质干细胞定向分化为胰岛样细胞存在可行性。Objective To explore the possibility of differentiating functional islet-like cells from marrow mesenchymal stem cells.Methods Rat marrow mesenchymal stem cells (MSC) were isolated from Wistar rats and cultured.Passaged MSC were induced to differentiate into islet-like cells under following conditions:LN group,pre-induced with L-DMEM containing 10 mmol/L Nicotinamide+1 mmol/L β-Mercaptoethanol+20 % FSC for 24 h,then induced with serum free H-DMEM solution including 10 mmol/L Nicotinamide+1 mmol/L β-Mercaptoethanol for 10 h; HN group,pre-induced with L-DMEM containing 20 mmol/L Nicotinamide+20 % FSC for 24 h,then induced with serum free H-DMEM solution including 10 mmol/L Nicotinamide for 10 h.Differentiated cells were observed under inverse microscopy.Results While MSC cells were typically fibrocyte-like,grape-like coherent clustered cells were observed under both LN and HN groups,which were similar to pancreatic islet cells.Some cells differentiated into neurocytes.Conclusion Functional islet-like cells can be differentiated from marrow mesenchymal stem cells,which may be a new procedure for clinical diabetes stem -cell therapy.

关 键 词：骨髓基质干细胞 胰岛样细胞 形态学观察 定向分化 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]