PTEN抑癌基因对高转移性黏液表皮样癌细胞系生物学行为的影响  

Effects of the Exogenous PTEN Tumor Suppressor Gene on Adhesion, Migration and Invasion of the Highly Metastatic Mucoepidermoid Carcinoma Cell Line

作　　者：刘斌[1] 吴军正[1] 司徒镇强[1] 

机构地区：[1]第四军医大学口腔医学院口腔生物教研室,西安720032

出　　处：《口腔医学》2004年第2期68-70,共3页Stomatology

基　　金：第四军医大学科技创新工程 (CX99A0 11)

摘　　要：目的　探讨外源野生型PTEN抑癌基因对人高转移性黏液表皮样癌细胞系M3SP2体外黏附、迁移和侵袭特性的影响。方法　用脂质体介导方法将PTEN抑癌基因导入M3SP2细胞系 (M3SP2 -PTEN细胞 ) ,转染空载体的细胞系作为对照(M3SP2 -pBp细胞 ) ,分别用四甲基偶氮唑蓝 (MTT)比色法、细胞迁移试验及细胞侵袭试验测定M3SP2 -PTEN细胞和M3SP2 -pBp细胞体外黏附、迁移和侵袭能力。结果　M3SP2 -PTEN细胞在Metrigel和Fn基质上黏附数量减少 ,与对照组比较差异有显著性 (P <0 0 1) ,黏附抑制率分别为 34 3%和 4 9 4 %。M3SP2 -PTEN细胞在Matrigel基质上迁移距离小 ,与对照组比较差异有显著性 (P <0 0 5 ) ,迁移抑制率为 2 6 0 %。M3SP2 -PTEN细胞体外侵袭细胞数量减少 ,与对照组比较差异有显著性 (P <0 0 1) ,侵袭抑制率为 31 4 %。结论　外源性PTEN抑癌基因对高转移性黏液表皮样癌细胞系M3SP2体外黏附、迁移和侵袭具有抑制作用。Objective To study effects of the exogenous wild-type PTEN tumor suppressor gene on in vitro adhesion, migration and invasion of the highly metastatic mucoepidermoid carcinoma cell line M3SP2. Methods The exogenous wild-type PTEN gene was transducted with lipofectamine into the M3SP2 cells. The exogenous PTEN expressing cells were named as M3SP2-PTEN, and the control vector transfected cells as M3SP2-pBp.The in vitro adhesion of M3SP2-PTEN and M3SP2-pBp cells on extracellular matrix fibnectin and matrigel was detected using MTT assay. The in vitro migration of M3SP2-PTEN and M3SP2-pBp cells on extracellular matrix matrigel was assayed with cellular migration test. The in vitro invasion of M3SP2-PTEN and M3SP2-pBp cells was determined by Transwell. Results The in vitro cell adhesion experiment showed that the exogenous PTEN gene in the M3SP2-PTEN cells decreased cell adhesion to matrigel and fibnectin extracellular matrix by 34.3% and 49.4%, respectively, compared with the control cells. It also revealed that migration of the M3SP2-PTEN cells on the matrigel was inhibited by 26.0%. In vitro invasion of the M3SP2-PTEN cells was significantly reduced by 31.4%. Conclusion The exogenous wild-type PTEN gene has significant effects of anti-adhesion, anti-migration, and anti-invasion on the highly metastatic mucoepidermoid carcinoma cell line M3SP2.

关 键 词：PTEN抑癌基因 黏液表皮样癌 黏附 迁移 侵袭 

分 类 号：R349.52[医药卫生—基础医学]