电化学发光PCR技术检测转基因植物  

Detection of Genetically Modified Plant by Electrochemiluminescence PCR Method

作　　者：刘晋峰[1] 邢达[1] 沈行燕[1] 朱德斌[1] 

机构地区：[1]华南师范大学激光生命科学研究所,广州510631

出　　处：《生物化学与生物物理进展》2004年第4期375-378,共4页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金面上资助项目 ( 60 3 780 43 ) ;广东省自然科学基金团队资助项目 ( 0 15 0 12 );广东省科技计划资助项目( 2 0 0 2C2 0 60 7)~~

摘　　要：随着转基因植物种类的增多 ,转基因植物的检测也成了当今的热门话题 .电化学发光法是将电化学与化学发光两种高灵敏度方法相结合 ,实现了检测的高效、准确、无毒害 .电化学发光PCR法首次将电化学发光技术、PCR技术和双探针杂交技术结合起来 ,用于检测CaMV (cauliflowermosaicvirus) 35S启动子 ,从而判断其是否含有转基因成分 .PCR产物与生物素标记的探针杂交 ,可以起到筛选的作用 ;与三联吡啶钌标记的探针杂交则可用于电化学发光检测 .两种探针同时与转基因样品PCR产物杂交 ,使结果避免假阳性的影响而更加准确 .实验表明 :此方法可以准确地检测到 35S启动子的存在 .该方法灵敏度高 ,可靠性强 ,操作简便 ,结果准确 ,有望成为一种高效的转基因检测方法 .With the rapid development of the genetically modified plant, more and more genetically modified plant food has been pouring into the market. It has been paid much attention to detection of GMP under the controversy of GMP safety. Electrochemiluminescence method is the chemiluminescent reaction of species that are generated electrochemically at the surface of an electrode. It is a high efficiency and accurate detection method. Electrochemiluminescence PCR method makes electrochemiluminescence technique, PCR technique and two nucleic acid probes hybridization technique band together for the first time. In this method, whether plant containing GM component by detection of CaMV (Cauliflower mosaic virus) 355 promoter are distinguished. The results show thus, it is indicate that, the method can detect genetically modified plant exactly. The method is simple, reliable and correctly for analysis of GMP.

关 键 词：转基因植物 电化学发光 PCR技术 CaMV35 S启动子 蛋白质 

分 类 号：Q943.2[生物学—植物学] Q503