卵粒玻片的制备和自制分样筛及线虫分离方法的改进  

Preparation of the Egg Granules Slide and Self-manufacturing the Sample Sieve and Improvement of the Method of Extracting Nematodes

作　　者：潘沧桑[1] 刘凡[1] 

机构地区：[1]厦门大学生命科学学院寄生动物研究室,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2004年第3期416-419,共4页Journal of Xiamen University：Natural Science

基　　金：福建省重大科技项目(2002N005)

摘　　要：介绍3种研究线虫用的小工具制作方法:(1)制备卵粒玻片供检测使用;(2)用PVC管和筛网加AB胶粘结自制分样筛;(3)小的分样筛与漏斗法结合从植物样品获得纯净的线虫.卵粒玻片有容易观察、制作方便和可供胚胎发育研究等优点.自制的分样筛不但分离效果好,而且经济实用,颇受使用者欢迎.The communication recommend the methods of manufacturing 3 small tool used for studying the plant nematodes: 1)preparation of nematode egg granules slide used for examining. 2)preparation of self-manufacturing sample sieve by using PVC tube and sieve-net adding AB glue. (3) Obtaining the pure nematodes from plant sample by using the small sample sieve combining funnel method. The egg granules slide was easy to observe and was convenient for manufacture and can be used for studying of embryo development et al. The self-manufactured sample sieves not only produced the excellent extracting effect, but also are economical and applied, therefore was welcomed by the user.

关 键 词：卵粒 分样筛 植物线虫 分离方法 传毒线虫 

分 类 号：S433[农业科学—农业昆虫与害虫防治]