甘蓝型油菜SSR、ISSR标记的遗传多样性及其与杂种表现的关系  

Relationship Between Hybrid Performance and Genetic Diversity Based on SSR and ISSR in Brassica napus L.

作　　者：沈金雄[1,2] 傅廷栋[3] 杨光圣[3] 

机构地区：[1]中国农业科学院油料作物研究所 [2]华中农业大学作物遗传改良国家重点实验室,武汉430070 [3]华中农业大学作物遗传改良国家重点实验室

出　　处：《中国农业科学》2004年第4期477-483,共7页Scientia Agricultura Sinica

基　　金：国家重大基础研究资助项目(2001CB10807);欧盟资助项目(IC-18-CT97-0172)

摘　　要：利用SSR、ISSR标记技术分析了3个甘蓝型油菜自交不亲和系及22个不同来源的父本品种的遗传多样性,及其与这些亲本按NCII法配制杂种的产量性状表现及杂种优势的关系。结果表明,基于遗传距离的UPGMA(非加权类平均法)聚类法可将25个亲本材料分为6组,SI-1300、SI-1320各为一组,来源于中国的2个父本品种为一组,SI-1310与来源于国外的其它品种分属另3组。这种分组结果与材料的来源及系谱关系基本一致。各组间亲本在产量性状、种子品质性状及物候期性状上存在明显差别。SSR、ISSR标记的遗传距离与杂种单株产量极显著正相关,但决定系数较小。SSR及ISSR标记遗传距离难以预测甘蓝型油菜杂种产量及其优势。To investigate the relationship between genetic distance (GD) and hybrid performance, SSR (microsatellite)and ISSR (intro-simple sequence repeat) were employed to detect the genetic diversity of 3 double low self-incompatiblelines and 22 male parent varieties in Brassica napus from different origins. Hybrids were produced in a NCII mating designby hand-pollination. Results indicated that 25 parent varieties (lines) could be divided into six groups by UPGMA (un-weighted pair group mathematics average) clustering based on GD. SI-1300 and SI-1320 could be singly clustered into onegroup, respectively. Male varieties from China could be divided into another group. SI-1310 and male varieties from foreigncountries could be divided into other three groups. The grouping was generally consistent with parental pedigrees andgeographical origins. Significant differences in yield, quality and phenological period traits were observed among theseparent groups. Although hybrid yield per plant showed significant positive correlation with genetic distance based on SSRand ISSR markers, but the determination coefficients (R2) were low. It appeared to be unsuitable for using the geneticdistance based on SSR and ISSR markers to predict heterosis and hybrid performance in Brassica napus.

关 键 词：甘蓝型油菜 SSR标记 ISSR标记 遗传多样性 杂种 产量性状 杂种优势 

分 类 号：S565.4[农业科学—作物学]