印楝种仁中印楝素微波萃取方法研究  

Microwave Extraction of Azadirachtin from Neem Seeds

作　　者：宗乾收[1] 林军[1] 张征[1,2] 武永昆 苏鹏娟 

机构地区：[1]云南大学应用化学系 [2]云南中科生物产业有限公司,昆明650106 [3]云南中科生物产业有限公司

出　　处：《农药》2004年第5期230-232,共3页Agrochemicals

基　　金：云南省技术创新人才基金 (2 0 0 1PY0 1 1 )资助项目

摘　　要：以家用变频微波炉改造而成的微波化学反应器为主要设备 ,研究了在微波诱导下萃取印楝种仁中印楝素的方法。对微波功率、辐射时间和萃取溶剂等影响微波萃取的条件进行了筛选 ,优化条件为以甲醇为萃取溶剂 ,微波功率 2 80W ,辐射时间 10 0s,溶剂用量 12 0ml。以HPLC法测定萃取物中的印楝素A含量 ,采用HypersilODS (4mm× 2 5 0mm)色谱柱 ,水 乙腈 (6 3∶37)为流动相 ,检测波长 2 18nm ,流速为 1ml/min。将微波萃取法与常规甲醇室温浸提法和加热回流法进行了比较 ,结果表明 ,微波诱导萃取法具有萃取速度快、溶剂用量少、萃取效率高等优点。Microwave extraction of azadirachtin from neem seeds was studied using a modified home microwave oven.The effects of power setting,irradiation time,solvent volume during extraction,and different solvents were tested.Microwave oven power set at 280 W,an irradiation time of 100 s,a solvent volume of 120 ml,and using methanol as the solvent provided optimum conditions. HPLC was used for determination of azadirachtin A.The column was a Hypersil ODS(4mm×250 mm),mobile phase was water/acetonitrile (63∶37),flow rate was 1 ml/min,and the UV detector was set at 218 nm.The microwave extraction method was compared to conventional maceration and extraction using methanol at room temperature followed by reflux.Microwave extraction compared favorably in being fast and efficient,using less solvent,and having a higher extraction yield.

关 键 词：印楝素 微波萃取技术 印楝种仁 辐射时间 HPLC 溶剂 生物活性 杀虫剂 

分 类 号：TQ4533[化学工程—农药化工]