高分辨气相色谱高分辨质谱测定鱼体中的类二噁英多氯联苯  

Determination of dioxin-like PCBs in fish by isotope dilution HRGC/HRMS

作　　者：姜杰[1] 张建清[1] 蒋友胜[1] 周健[1] 张红宇[1] 林凯[1] 

机构地区：[1]深圳市疾病预防控制中心,广东518020

出　　处：《中国卫生检验杂志》2004年第2期155-156,共2页Chinese Journal of Health Laboratory Technology

摘　　要：〔目的〕采用高分辨气相色谱 高分辨双聚焦磁式质谱联用仪 (HRGC HRMS)定量检测了鱼体中 12种类二口恶英多氯联苯 (PCBs)化合物。〔方法〕参照美国EPA1668A方法 ,采用索式抽提和自动纯化系统 (FMS)等仪器对鱼体样品中的类二口恶英PCBs进行纯化和富集、用同位素稀释气相色谱与高分辨质谱联用 -多离子检测方法检测。〔结果〕该方法可对PCBs同系物进行有效分离 ,同位素标准物的回收率分布于 44 .8%～ 93 .1%之间 ,CSL考核样的检测结果均在令人满意的范围之内 ,7种类二口恶英PCBs在鱼体中可检测出 ,总TEQ值为 0 .3 5pg g。〔结论〕该方法可以准确检测样品中类二口恶英PCBs ,所检鱼体中含有一定量类二口恶英PCBs ,有必要做更多样品 ,对其污染情况做出评价。Objective To determine dioxin-like PCBs in fish by HRGC/HRMS.〔Methods〕 According to EPA1668A,dioxin-like PCBs were extracted from fish by Soxhlet extraction,cleaned up by FMS and quantified by HRGC/HRMS-MID using isotope dilution methodology.〔Results〕 The congeners of PCBs could be separated well by this method.The recovery rate was 44.8%-93.1%.The result of CSL samples was in the range of certified values.Seven congeners of dioxin-like PCBs could be measured in the fish samples and the total toxicity equivalency quantity of the fish samples was 0.35pg/g.〔Conclusions〕 Dioxin-like PCBs in the fish samples can be accurately measured by this method.It is necessary to measure more fish samples to evaluate the contamination of fish samples in China.

关 键 词：高分辨气相色谱 高分辨质谱 鱼体 类二噁英多氯联苯 同位素 

分 类 号：O657.63[理学—分析化学]