小鼠骨髓前体细胞来源的树突状细胞超微结构  

Ultrastructure of Murine Bone Marrow-derived Dendritic Cells

作　　者：吴淑燕[1] 古涛[2] 周桓[2] 王响英[1] 李苏安[1] 毛棣华[1] 朱一蓓[2] 陈成[2] 周时勇[2] 张学光[2] 

机构地区：[1]苏州大学基础医学系电镜室,苏州215007 [2]苏州大学生物技术研究所,苏州215007

出　　处：《上海实验动物科学》2004年第1期34-36,60,共4页Shanghai Laboratory Animal Science

摘　　要：无菌制备BALB/c小鼠骨髓细胞,按常规方法分离纯化出树突状细胞(DCs),采用GM-CSF和IL-4诱导后,以凋亡肿瘤细胞负载未成熟DCs,再分别加入mCD40L-CHO细胞和TNF-α继续培养48h,按常规方法分别制备各发育阶段DCs的超薄切片。用透射电镜观察小鼠DCs在不同发育阶段的超微结构特征,并比较凋亡肿瘤细胞负载的小鼠DCs被CD40L和TNF-α刺激后超微结构的差别。实验结果证实DCs在分化发育成熟中存在异质性;DCs可通过吞噬凋亡的肿瘤细胞负载抗原;CD40配基化对DCs的分化成熟作用优于TNF-α。Dentritic cells (DCs) and their progenitors from murine bone marrow were collected and induced in vitro by both granulocyte-macrophage colony-stimulating (GM-CSF) and interleukin-4 (IL-4) for 5~6 days. Immature DCs were loaded with apoptotic tumor cells (AP-DC), then AP-DC vaccines were induced further maturation stimulated with MCD40L-CHO cells and TNF-αrespectively for 48 h. These DCs' morphology was observed under transmission electron microscropy. We found that immature DCs showed a few short and blunt cytoplasmic processes, there were specific morphology granules which liked earphone in some cells; the immature DCs had a powerful ability to capture antigens from apoptotic tumor cells in 5~6 th culturing day, the DCs engulfing the apoptotic bodies were observed; sub-cellular structures between CD40 ligation and TNF-αstimulated DCs were different, the former had typical morphology of DCs which exhibited many dendritic protrusions and the nucleus was irregular in shape, the cytoplasm contained numerous mitochondria, ribosomes, well-developed Golgi complexes, but scantly lysosomes. In addition, DCs might be in contact with each other. The results suggested that DCs were heterogeneous; DCs can acquire antigen by phagocytosing apoptotic bodies; CD40 ligation and the associated signal transduction played an essential role in myeloid DCs differentiation and maturation.

关 键 词：小鼠 骨髓前体细胞 树突状细胞 超微结构 CD40 抗原负载 

分 类 号：R392[医药卫生—免疫学]