离子对高效液相色谱法测定复方甲硝唑片中甲硝唑和维生素B_6含量  

Determination of metronidazole and vitamin B_6 in compound metronidazole tablets by ion-pair HPLC

作　　者：王自然[1] 伏光华 

机构地区：[1]山东省临沂师范学院农林学院动科系,山东临沂276003 [2]山东省临沂市药品检验所,山东临沂276001

出　　处：《中国兽药杂志》2004年第4期14-16,共3页Chinese Journal of Veterinary Drug

摘　　要：　建立了同时测定复方甲硝唑片中甲硝唑和维生素B6含量的离子对HPLC方法。采用ODS色谱柱,流动相为磷酸二氢钾和庚烷磺酸钠水溶液(每1L中含磷酸二氢钾13.6g与庚烷磺酸钠1.0g)-甲醇-三乙胺(85∶15∶0.4),用磷酸调pH至2.8;检测波长290nm,以峰面积计算,外标法定量。甲硝唑和维生素B6的线性范围分别为100～300μg/mL(r=0.9999)和5～50μg/mL(r=0.9999);平均回收率分别为100.2%(RSD=1.0%,n=6),99.8%(RSD=1.2%,n=6)。该方法测定结果准确,操作简便,回收率高。An ion-pair HPLC method for determination of metronidazole and vitamin B_6 in compound metronidazole tablets was establish. Metronidazole and vitamin B_6 were detected with an ODS column and 0.1 mol/L KH_2PO_4-methanol-triethylamine(85∶ 15∶ 0.4) as the mobile phase at 290 nm. The linear ranges of standard curve were 100～300 μg/mL (r=0.999 9) for metronidazole and 5～50 μg/mL (r =0.999 9) for vitamin B_6 , respectively. The average recoveries were 100.2%(RSD=1.0%) for metronidazole and 99.8%(RSD=1.2%) for vitamin B_6(n=6) The method is accurate and simple with high recoveries.

关 键 词：离子对高效液相色谱法 复方甲硝唑片 甲硝唑 维生素B6 溶液 

分 类 号：S859.2[农业科学—临床兽医学]