鸡痘鹌鹑化弱毒在犊牛睾丸细胞上的适应性试验  

Tests on the adaptation of quail adapted fowlpox virus to the bovine testicular cells

作　　者：王雅华[1] 裴春生 王伟利 谭守国 王东升 杨鹤松 

机构地区：[1]辽宁农业职业技术学院辽宁熊岳,115214 [2]吉林省生物制品厂,吉林吉林132101 [3]吉林省商检局,吉林长春130000

出　　处：《中国兽药杂志》2004年第4期36-37,共2页Chinese Journal of Veterinary Drug

摘　　要：　鸡痘鹌鹑化弱毒在犊牛睾丸细胞上培养传至7代,各代次均产生程度不同的致细胞病变作用,经测定至第6代病毒收获液在犊牛睾丸细胞(BTC)上的致病变作用(CPE)最明显,细胞产毒效价最高可达105.5EID50/0.2mL。另外,对7个代次的病毒液进行的安全试验结果表明,该病毒收获液安全有效,符合制苗标准。此试验为用BTC生产鸡痘活疫苗提供了重要依据。Quails adapted fowlpox virus was cultured seven generations in the bovinel testicular cells. Every generation of the virus could lead to CPE in the cells. At the 6th generation, the CPE was very obvious and the virus titer could get 10^(5.5) EID_(50)/0.2 mL. Meanwhile, the liquid with virus of seven generations were all tested for (security). The results showed that the live attenuated virus adapted to the BTC were safe as vaccine. The tests provided an important basis for producing live vaccine of fowlpox with BTC.

关 键 词：鸡痘病毒 犊牛睾丸细胞 BTC 适应性 效价 活疫苗 

分 类 号：S858.315.3[农业科学—临床兽医学] S859.797[农业科学—兽医学]