RP-HPLC法测定怀槐中染料木素和芒柄花黄素含量  

RP-HPLC determination of genistein and formononetin in various parts of Maackia amurensis

作　　者：黄文哲[1] 韦佳[1] 王峥涛[1] 周荣汉[1] 

机构地区：[1]中国药科大学,江苏南京210038

出　　处：《植物资源与环境学报》2004年第2期60-61,共2页Journal of Plant Resources and Environment

摘　　要：A method for the determination of genistein and formononetin in Maackia amurensis Rupr. et Maxim. by HPLC was reported. The RP-HPLC methods were used as follows: Hypersil ODS C18 column; The mobil phase is MeOH-1% acetic acid solution（gradient）, genistein and formononetin were determined by UV detector at 261nm and 249nm respectively. The results showed that average recovery and precision （RSD） for genistein and formononetinwere 100.4%,\ 101.8%and2.65%, 2.16% respectively. The method is appropriate for determinaton of genistein and formononetin in M. amurensis.A method for the determination of genistein and formononetin in Maackia amurensis Rupr. et Maxim. by HPLC was reported. The RP-HPLC methods were used as follows: Hypersil ODS C_(18) column; The mobil phase is MeOH-1% acetic acid solution(gradient), genistein and formononetin were determined by UV detector at 261nm and 249nm respectively. The results showed that average recovery and precision (RSD) for genistein and formononetinwere 100.4%,\ 101.8%and2.65%, 2.16% respectively. The method is appropriate for determinaton of genistein and formononetin in M. amurensis.

关 键 词：RP-HPLC法 怀槐 染料木素 芒柄花黄素 反相高效液相法 肿瘤细胞 

分 类 号：R284[医药卫生—中药学] Q946.8[医药卫生—中医学]