惊厥和亚惊厥剂量戊四唑对大鼠脑内NMDA受体亚单位蛋白表达的影响  

Effects of pentylenetetrazol at subconvulsant and convulsant dose on NMDA receptor subunits in rats

作　　者：朱丽君[1] 王敏珍[1] 吴晓华[2] 徐淑君[1] 罗建红[1] 陈忠[1] 

机构地区：[1]浙江大学医学院神经生物学实验室,浙江杭州310031 [2]浙江省金华市中心医院,浙江金华321000

出　　处：《浙江大学学报（医学版）》2004年第3期213-215,224,共4页Journal of Zhejiang University(Medical Sciences)

基　　金：国家"973"计划基金( G19990 5 4 0 0 3);国家自然科学基金( 30 3716 38);浙江省青年人才基金( R30 3779)资助项目

摘　　要：目的 :研究不同剂量戊四唑刺激对大脑皮层内 N-甲基 - D-天门冬氨酸 ( NMDA)受体 NR1、NR2 A和NR2 B 3种亚单位表达量的影响。方法 :腹腔注射亚惊厥剂量 ( 35 mg/kg)和惊厥剂量 ( 5 0 mg/kg)的戊四唑 ,注射后不同时间点分离大脑皮层 ,用免疫印迹法检测 NMDA受体 NR1、NR2 A、NR2 B 3种亚单位的蛋白含量。结果 :注射戊四唑亚惊厥剂量和惊厥剂量组大鼠的行为学差异非常显著 ( P<0 .0 0 1) ,但 NMDA受体 3种亚单位仅 NR2 A亚单位在 1h点有显著性增高 ,且惊厥剂量组 NR2 A的表达量比亚惊厥剂量组高 ( P<0 .0 5 )。惊厥剂量组 NR2 A和NR2 B表达有先增加再降低然后恢复至正常水平的趋势 ,而 NR1亚单位改变不明显。结论 :戊四唑引起惊厥的机制可能与戊四唑首先影响 NR2亚单位蛋白的表达 ,以调节Objective: To investigate the expression of N-methyl-D-asparate (NMDA) receptor subunit proteins after administration of different doses of pentylenetetrazol (PTZ). Methods: After ip injection of a subconvulsant (35 mg/kg) and convulsant (50 mg/kg) dose of PTZ, the rats were decapitated at different time points. The levels of cortical NR1,NR2A and NR2B subunit proteins were detected by immunoblotting. Results: 35 mg/kg PTZ and 50 mg/kg PTZ elicited different behavioral changes ( P <0.001). The NR2A subunit in the cortex significantly increased 1 h after PTZ injection ( P <0.05). For the 50 mg/kg group, both the NR2A and NR2B subunits proteins increased at 1 h in the cortex and then decreased; the protein levels returned to normal after 48 h. However, NR1 subunit had no changes. Conclusion: The NR2 subunit is involved in PTZ-induced seizure.

关 键 词：戊四唑/投药和剂量 受体 N-甲基-D-天冬氨酸/分析 

分 类 号：R96[医药卫生—药理学]