酵母双杂交筛选与视黄醇结合蛋白有相互作用的蛋白质  

The Screen of Interaction Protein with RBP Using Two-hybrid System

作　　者：王世春[1] 刘云[1] 李永辉[1] 徐琪寿[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850

出　　处：《科学技术与工程》2004年第6期462-466,共5页Science Technology and Engineering

基　　金：国家自然科学基金 ( 3 0 170 80 1)资助

摘　　要：为了研究视黄醇结合蛋白 (RBP)在维生素A代谢调控中的作用和与相关疾病发生、发展的关系 ,以RBP作为诱饵基因构建了融合表达载体pGBKT7 RBP ;在排除自身转录激活活性的基础上 ,与人肾脏cDNA预转库融合筛选 (PretransformedMATCHMAKERLibraries) ,经营养缺陷型和X α gal显色鉴定 ,从中筛选出三种与RBP有相互作用的新蛋白质 。On the basis of having constructed inducing fusion vector: pGBKT7-RBP, pretransformed human kidney cDNA library PACT2 and pGBKT7-RBP were co-expressed. After libraries screening and nutritional selection as well as X-α-gal analysis, three protein sequence of putative interaction were obtained. And complete sequences were cloned with PCR application from human testis epidermis cDNA library.

关 键 词：视黄醇结合蛋白 相互作用 酵母双杂交 维生素A 基因克隆 

分 类 号：Q513[生物学—生物化学]