间接EL ISA与HI检测鸡新城疫抗体相关性  

The Comparison of Testing Newcastle disease virus Antibody by Indirect ELISA and HI

作　　者：陈福勇[1] 吴玉苹[1] 曹红[1] 白江 陈晨[1] 白如念[1] 庞平[1] 

机构地区：[1]中国农业大学动物医学院,北京100094 [2]海博莱生物科技有限公司,北京101500

出　　处：《动物医学进展》2004年第3期88-90,共3页Progress In Veterinary Medicine

摘　　要：应用间接 EL ISA和微量红细胞凝集抑制试验 ,同时对来自两个鸡场的 1 71份血样进行常规鸡新城疫抗体检测。结果表明 ,二种方法的抗体效价检测结果呈显著相关 ,其相关系数为 0 .92 49(r2= 0 .8560 ) ,设置 t检验 ,差异显著 t<0 .0 0 0 1 ,(n= 1 71 ) ,并测得总体和各鸡群的回归方程 ,其中总体的回归方程式为 EL ISA =0 .2 80 5× HI-0 .450 9。 EL ISA方法具有敏感 ,特异 ,快速 ,简便的优点 。The antibody response of commercial chickens vaccinated for Newcastle disease virus antibodies was assayed by micro-haemagglutination inhibition (HI) and indirect enzyme-linked immunosorbent assay (ELISA). The latter test was performed using LABORATORIOS HIPRA,S.A.' CIVTESTTM AVI NDV and the results from the two methods compared. Samples of 171 chickens from two farms were assayed.A correlation efficiency was 0.9249(r^2=0.8560), based on individual data, more accurately reflected the correlation between the two methods.The regression equations for the overall and the individual groups were given, of which the overall regression effciecy was ELISA=0.2805×HI-0.4509 (t<0.0001). Thus the ELISA values proved to be significantly correlative with the HI values. Furthermore the ELISA has sensitive,specific, rapid serologic diagnosis of viral diseases and is thus a better method for the analysis of both a large quantity of samples as well as trace samples.

关 键 词：间接ELISA 鸡 新城疫 抗体检测 相关性 HI试验 

分 类 号：S858.31[农业科学—临床兽医学]