近交系大鼠STR复合扩增DNA多态性的分析  

Analyzsis of DNA Polymorphism for Inbred Rat Using STR-PCR

作　　者：李瑞生[1] 陈振文[1] 张嘉保[2] 孙岩松[1] 

机构地区：[1]军事医学科学院实验动物中心,北京100071 [2]解放军军需大学动科系繁育教研室,吉林长春130062

出　　处：《动物医学进展》2004年第3期107-109,共3页Progress In Veterinary Medicine

基　　金：总后卫生部基金课题资助 (98M116 )

摘　　要：实验从已筛选出的近交系大鼠的 2 0个基因座位点中 6个位点 ,合成 6对引物 ,利用聚合酶链反应 (PCR)扩增技术对国内已知的SHR、SHRSP、L EW、RCS、WKY和 F3 44等 6个近交系大鼠各 4只进行了 DNA多态性的分析。结果表明同一品系不同个体之间没有多态性 ;不同品系个体之间具有显著多态性 ;利用这6个特异性的位点对国内 6种常用近交系大鼠可有效地进行遗传背景的鉴别。该方法能有效地对近交系与杂交系、品系与品系、品系与亚系加以区分 ,并大大地提高了其监测效率。因此 ,本实验为实验动物遗传背景的监测提供一个高效快捷。In the experiment,It were selected 6 specific loci from studyed 20 loci of inbred strain rats.DNA polymorphism of 6 inbred strain rats (SHR、SHRSP、WKY、LEW、RCS、F344) were analyzed using polymerase chain reaction(PCR).The results indicated that there was not polymorphism between different individuals;there were more polymorphisms between different groups.It can much distinguish genetic background of national 6 groups of inbred strain rats using 6 specific loci. The method of PCR-analyzed can be used for distinguishing between inbred and outbred strains,different strains,strain and substrain. and it improved more monitoring efficiency. And so it provided a speedy、convenient、accurate method for genetic monitor of laboratory animals in the experiment.

关 键 词：近交系大鼠 STR基因座 DNA多态性 PCR 实验动物 

分 类 号：S865.1[农业科学—野生动物驯养]