小白菜中残留虫酰肼的超临界流体萃取条件的研究  

Investigation on Extraction Conditions of Tebufenozide Residue from Cabbage Using Supercritical Fluid Extraction

作　　者：杨立荣 张兴[1] 陈安良[1] 李广泽[1] 

机构地区：[1]西北农林科技大学无公害农药研究服务中心

出　　处：《色谱》2004年第3期263-266,共4页Chinese Journal of Chromatography

基　　金：国家"948"引进项目资助(项目编号:971059).

摘　　要：将超临界流体萃取(supercriticalfluidextraction,SFE)技术与高效液相色谱分析相结合,建立了特异性杀虫剂虫酰肼的萃取分离方法。SFE对虫酰肼的萃取条件:压力48.3MPa(7000psi),温度60℃,静态萃取时间20min,CO2体积10mL,改性剂甲醇添加量0 04mL/g,丙酮为收集溶剂。在此条件下,SFE对虫酰肼的萃取率为100 75%,所得样品可直接用于高效液相色谱分析。色谱条件:紫外 二极管阵列检测器(检测波长为245nm),C18键合色谱柱,乙腈 水(体积比为55∶45)流动相,流速1 0mL/min,柱温25℃,进样量5μL。An extraction method of tebufenozide using supercritical fluid extraction (SFE) and high performance liquid chromatography (HPLC) was developed. The selected conditions were 48.3 MPa ((7 000) psi), 60 ℃, 20 min of static time, dynamic extraction with 10 mL of CO_2, 0.04 (mL/g) of methanol as static modifier, and 5 mL of acetone as collecting solvent. Under these conditions, the recovery of tebufenozide extracted by SFE was 100.75%. The extract was analyzed directly by HPLC with a photodiode array detector. The chromatographic conditions were: UV detection wavelength, 245 nm; column, a C_(18) column; mobile phase, acetonitrile-water (55∶45, v/v); flow rate, 1.0 mL/min; injection volume, 5 μL.

关 键 词：超临界流体萃取 高效液相色谱法 虫酰肼 残留 小白菜 

分 类 号：O658[理学—分析化学]