苦皮藤素人工抗原的合成与鉴定  

Synthesis and characterization of artificial antigen of Celangulin Ⅴ

作　　者：杨润亚[1] 祁志军[1] 吴文君[1] 

机构地区：[1]西北农林科技大学农药研究所

出　　处：《西北农林科技大学学报（自然科学版）》2004年第6期64-68,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(30130130);烟台师范学院中青年自然科学基金项目(000443)

摘　　要：　采用琥珀酸酐法合成了苦皮藤素 的衍生物苦皮藤素 -半琥珀酸酯(CA-SG),在此基础上,分别采用混合酸酐法和碳化二亚胺法合成了苦皮藤素 的人工抗原(CA-BSA,CA-BSA2),采用紫外分光光度计法、SDS-PAGE及PAGE电泳法对合成的人工抗原进行了定性鉴定,结果均表明人工抗原合成成功。根据半抗原、人工抗原及其载体在240nm处的紫外吸光值,计算出2种人工抗原中半抗原与载体的分子结合比率分别为11.6和10.7。The primarily study on the immunoassay of Celagulin Ⅴ,one kind of insecticidal compound from plants,was carried out.Firstly,the derivative of Celangulin Ⅴ,called as CA-SG,was synthesized by succinic anhydride method;Then,two artificial antigen of Celangulin Ⅴ-CA-BSA and CA-BSA2 were prepared by mixed anhydride method and carbodiimide method respectively,The characterization of hapten-protein conjugates were examined by three methods,ultraviolet spectrophotometry,sodium dodecyl sulfate-polyacrylamide gel electrophoresis and polyacrylamide gel electrophoresis respectively.The number of hapten molecules per molecule of protein estimated by ultraviolet spectrophotometry according to the density of compounds at 240 nm for two conjugates were 11.6 and 10.7 respectively.

关 键 词：苦皮藤素V 人工抗原 合成 鉴定 混合酸酐法 碳化二亚胺法 半抗原 植物源农药 

分 类 号：S482.39[农业科学—农药学]