奶牛乳腺炎分离细菌裂解苗免疫试验  

Immunization with lysate bacteria isolated from mastitis bovine milk

作　　者：王赟[1] 靳亚平[1] 林炜明[1] 利光辉[1] 陈秀荔[1] 

机构地区：[1]西北农林科技大学家畜生殖内分泌与胚胎工程农业部重点开放性实验室,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2004年第6期32-34,40,共4页Journal of Northwest A&F University(Natural Science Edition)

摘　　要：　应用自奶牛乳房炎检测为阳性的牛乳汁中分离的4种优势G+菌,分别与葡聚糖、油佐剂和FIA3种佐剂制成细菌裂解苗免疫家兔。于免疫后第7天开始,每隔7d采集心脏血,分离血清,以试管凝集试验检测血清中抗体效价,以此评价所分离细菌的免疫原性和所用佐剂的免疫促进作用,筛选适宜的佐剂。结果显示,所分离的优势G+菌刺激家兔机体后可产生高效价的抗体,免疫后第7天,抗体效价开始明显上升,第28天左右达到峰值,并一直持续到试验结束(63d)。比较3种佐剂的免疫促进效果,以FIA效果最好,但3种疫苗间的免疫效果差异不显著,而与对照组相比,差异均达极显著(P<0.01)。4 kinds of predominant G^+ bacteria isolated from mastitis bovine milk were used to prepare lysate vaccine mixed with 3 sorts of adjuvants.After immunization,the rabbits blood samples were collected every 7 d from 7th day postimmunization.In order to determine if these 4 kinds of predominant G^+ bacteria have better antigenicity and select proper adjuvant,we collected sera to determine agglutination titer by agglutination reaction test tube.The result revealed,agglutination titer has arised after immunization and increased to peak about 28th day postimmunization.It sustained at high lever at 63rd day postimmunization.Adjuvant C is the best among these 3 sorts of adjuvants,but there are no difference in statistics.Compared to control group,all these 3 kinds of adjuvants are significantly different in statistics ((P<0.01)).

关 键 词：奶牛 乳腺炎 分离细菌裂解苗 免疫佐剂 免疫原性 疫苗 

分 类 号：S858.23[农业科学—临床兽医学] S852.52[农业科学—兽医学]