喜树叶片硝酸还原酶活性的测定方法  

Determination Method of Nitrate Reductase Activity in the Leaves of Camptotheca acuminata

作　　者：孙世芹[1] 阎秀峰[1] 

机构地区：[1]东北林业大学,哈尔滨150040

出　　处：《东北林业大学学报》2004年第3期83-84,共2页Journal of Northeast Forestry University

基　　金：国家自然科学基金 (30 0 70 0 86);黑龙江省杰出青年基金 (JC- 0 2 - 1 1 )资助项目

摘　　要：以喜树 (Camptothecaacuminata)为材料 ,探讨了用体内法测定其叶片硝酸还原酶活性的若干问题 ,比较分析了硝酸盐诱导、2 ,4 -二硝基苯酚、三氯乙酸及煮沸等因素对硝酸还原酶活性测定结果的影响 ,以寻求体内法测定喜树叶片硝酸还原酶活性的最佳条件。试验表明 ,体内法是一种操作简便、准确可靠的测定喜树叶片硝酸还原酶活性的方法 ,为进一步研究喜树的氮代谢奠定了基础。By using Camptotheca acuminata as experimental materials, the determination of the nitrate reductase activity (NRA) in the leaves of Camptotheca acuminata was discussed by in vivo method. The effects of several main factors of in vivo method such as nitrate induction, 2,4-dinitrophenol (DNP), trichloroacetic acid (TCA) and boiling etc. on the determination of the NRA were compared and analyzed. The modified in vivo method, which is reliable and easy to be manipulated, is feasible to determine the NRA in the leaves of C. acuminata.

关 键 词：喜树 硝酸还原酶 体内法 

分 类 号：S792.99[农业科学—林木遗传育种] Q946.5[农业科学—林学]