氯胺酮对He-Ne激光照射促人正常皮肤成纤维细胞增殖作用的影响  

Effects of Ketamine in Combination with 468.7 mJ/cm^2 He-Ne Laser Irradiation on Proliferation in Human Skin Fibroblasts

作　　者：李燕[1] 角建瓴[2] 刘承宜[1] 洪岸[3] 刘颂豪[4] 

机构地区：[1]华南师范大学激光运动医学实验室,广东广州510631 [2]暨南大学中医系,广东广州510632 [3]暨南大学生物工程研究所,广东广州510632 [4]华南师范大学信息光电子学院,广东广州510631

出　　处：《中国激光》2004年第4期509-512,共4页Chinese Journal of Lasers

基　　金：美国激光医学会2001年度暑期资助项目;国家自然科学基金(60178003和6027812);广东省自然科学基金团队项目(20003061);重点项目(200114 80);华中科技大学激光技术国家重点实验室开放基金;广东省"千百十工程"优秀人才培养基金(Q02087)资助项目

摘　　要：针对动物模型伤口的光生物调节作用 ,用He Ne激光对人正常皮肤成纤维细胞增殖的促进作用作为细胞模型 ,研究了麻醉药氯胺酮的影响。用MTT法测定细胞增殖作用 ,分别研究了 1 5 6mW/cm2 He Ne激光 ( 0 ,10 ,30 ,10 0和 30 0s照射 )对人正常皮肤成纤维细胞的增殖作用和氯胺酮 ( 0 ,0 6 ,1 2和 2 0 μg/mL)预处理对He Ne激光 ( 30 0s照射 )细胞增殖作用的影响。氯胺酮 ( 0 6 ,1 2 ,2 0 μg/mL)预处理能抑制 4 6 8 7mJ/cm2 He Ne激光促人正常皮肤成纤维细胞增殖的作用。这说明动物实验中用于麻醉的药物可能对激光促进伤口愈合的实验结果有影响。Based on the photobiomodulation on wound healing of animal models, the stimulative effects of He Ne laser irradiation on proliferation of human normal skin fibroblasts were used as the cell model to investigate the role of ketamine as an anesthetics in animal model experiments in this paper. The cell proliferation was measured using MTT based colorimetric assay. The effects of 1.56 mW/cm 2 He Ne laser irradiation on human normal skin fibroblasts for 0, 10, 30, 100, 300 s, and on the cells preincubated with different concentrations of ketamine (0, 0.6, 1.2, 2.0 μg/mL) for 300 s were investigated, respectively. The results show that human normal skin fibroblast proliferation induced by He Ne laser irradiation (468.7 mJ/cm 2) was inhibited when the cells were preincubated with different concentrations of ketamine (0.6, 1.2, 2.0 μg/mL). These results suggest that anesthetics may affect photobiomodulation on wound healing.

关 键 词：激光技术 成纤维细胞 低强度激光 氯胺酮 

分 类 号：R318.51[医药卫生—生物医学工程]