6-羧基荧光素琥珀酰亚胺酯的合成  

Synthesis of 6-carboxy flouorescein succinimidyl Ester

作　　者：彭正红[1] 彭孝军[1] 

机构地区：[1]大连理工大学精细化工重点实验室,大连116012

出　　处：《染料与染色》2004年第2期93-94,共2页Dyestuffs and Coloration

基　　金：国家自然科学基金资助项(20376010)

摘　　要：在甲基磺酸溶剂中,间苯二酚和偏苯三甲酸酐反应,得到6-和5-羧基荧光素异构体的混合物,反应产物与特戊酰氯,二异丙胺反应,分离得到带有二特戊酰基保护的6-羧基荧光素二异丙胺盐,水解后酯化,得到6-羧基荧光素琥珀酰亚胺酯。Synthesis of active 6-carboxyfluorescein succinimidyl esters was described. Resorcinol was condensed with trimellitic acid in methylsulfonic acid as a solvent to give a mixture of 5-(6-)carboxyfluorescein isomers. The obtained carboxyfluorescein was then reacted with pivalyl chloride and di-isopropy lamine, the purified 6-carboxyfluorescein dipivalate di-isopropy lamine salt was separated. After hydrolysis, tne product of 6-carboxyfluorescein dipivalate di-isopropylamine salt was to be esterized to give the active ester.

关 键 词：荧光素 异构体 特戊酰氯 二异丙胺 水解 

分 类 号：TQ617.2[化学工程—精细化工]