应用柱凝集技术进行Rh血型抗原分型的研究  

STUDY OF RH NEGATIVE GROUP GENE IDENTIFICATION WITH COLUMN AGGLUTINATION TECHNOLOGY

作　　者：张春来 陈晓建 于咏梅 谷彦霞 陈召江 杨振中 

机构地区：[1]临沂市红十字会中心血站,山东临沂276000

出　　处：《临沂医学专科学校学报》2004年第2期95-97,共3页Journal of Linyi Medical College

基　　金：临沂市科技发展计划项目 (No .0 3 3 0 163 )

摘　　要：目的　 探讨应用柱凝集技术进行Rh血型抗原分型的价值。方法　 采用博唯优柱凝集系统对RhD( )献血员进行Rh血型抗原分型 ,对弱D型进行确认 ,并进行抗体筛选、鉴定。结果　 共筛出RhD( )献血员 5 0 9例 ,Rh血型抗原分布频率ccdee >Ccdee >ccdEe >CCdee >CcdEe >ccdEE ,基因频率分布cde >Cde >cDE。筛出弱D型 16例 ,弱e型 1例。发现抗 D 7例 ,抗 M 2例 ,抗 c 1例。结论　 柱凝集技术可有效应用于Rh血型抗原分型 ,对建立和完善Rh稀有血型库有重要价值。Objective To evaluate the application of column agglutination technology in Rh blood group gene identification.Methods The column agglutination technology was used in the identification of Rh negative dornors,the classification of Rh antigen,the determination of weak type D,and the identification of the antibodies.Results 509 were initially selected as Rh negative.The distribution frequencies were as fottows:ccdee>Ccdee>ccdEe>CCdee>CCdEe>ccdEE,while the gene distribution frequencies were as follows:cde>Cde>cDE.Conclusion The column agglutination technology can be used effectively in the identification of Rh negative group.And it is valuable in the construction and improvement of rare blood bank.

关 键 词：柱凝集技术 RH血型 抗原 分型 

分 类 号：R457.11[医药卫生—治疗学]