氧化苦参碱抗大鼠肝纤维化及其免疫调控作用  

Immunomodulating Effects of Oxymatrine Therapy in Rats with Hepatic Fibrosis

作　　者：余小虎[1] 朱金水[1] 俞华芳[1] 朱励[1] 

机构地区：[1]上海交通大学附属第六人民医院消化科,上海200233

出　　处：《中国临床医学》2004年第2期163-165,共3页Chinese Journal of Clinical Medicine

摘　　要：目的:探讨氧化苦参碱抗肝纤维化及其免疫调控机理。方法:采用皮下注射四氯化碳及饮用10％酒精的方法使Wistar大鼠产生肝纤维化,肝纤维化大鼠随机分成二组,分别为自然恢复组(R)和氧化苦参碱治疗组(T),免疫组化法检测肝组织中免疫指标TNFα、IL-2、IL-10、IFNγ与纤维化指标Ⅰ、Ⅲ、Ⅳ型胶原及TGFβ1的水平。结果:肝纤维化大鼠肝组织TNFα与IL-10含量明显增高,而IL-2与IFNγ则明显下降;氧化苦参碱治疗9周后,在纤维化指标明显降低的同时,上述细胞因子的异常亦明显纠正。结论:氧化苦参碱有显著逆转肝纤维化作用,其机制可能与机体免疫调节功能有关。Objective: to investigate the effects of oxymatrine on hepatic fibrosis and its immune modulation. Methods:hepatic fibrosis was developed in Wistar rats within 8 weeks using subcutaneous injection of carbon tetrachloride (twice a week, 3ml·kg-1) and drunk alcohol (containing 10% ethanol), then the rats with hepatic fibrosis were randomly divided into two groups receiving intraperitoneal injection of oxymatrine(100mg·kg-1·d-1, T group ) and normal saline(R group) respectively. The duration of treatment was scheduled 9 weeks, then the levels of TNF α、IL-2、IL-10、IFNγ、collagen type Ⅰ、collagen type Ⅲ、collagen type Ⅳ and TGFβ1 in liver tissue were measured using immunohischemistry staining at the end point. Results: the levels of TNF α and IL-10 in rats with hepatic fibrosis were obviously higher than that in normal rats, whereas the levels of IL -2 and IFNγ were markedly decreased, after the therapy of oxymatrine, with the severity of hepatic fibrosis was reduced, the abnormity of all the above cytokines were markedly improved too. Conclusion: oxymatrine has obvious reversal effects on rats with hepatic fibrosis, it may be correlated with immunologic regulation in rats.

关 键 词：氧化苦参碱 大鼠 肝纤维化 免疫调控 四氯化碳 药物治疗 

分 类 号：R259.752[医药卫生—中西医结合] R285.5[医药卫生—中医内科学]