支气管哮喘遗传因子研究  

Studies on the Genetic Diathesis of Asthma Bronchial

作　　者：张瑛琪[1] 孙白云[1] 戴建军[2] 武守山[2] 张岸平[2] 赵沉浮[2] 宁树成[2] 

机构地区：[1]唐山工人医院呼吸内科,唐山063000 [2]华北煤炭医学院生物科学系,唐山063000

出　　处：《遗传》2004年第2期147-150,共4页Hereditas（Beijing)

基　　金：河北省科技厅科技攻关项目资助(022761106)~~

摘　　要：研究谷胱甘肽 S 转移酶(glutathioneS transferase,GST)M1和T1基因多态性与支气管哮喘(asthmabronchial)的关系。采取聚合酶链反应对60名支气管哮喘患者和60名正常对照进行了GSTM1和GSTT1基因非缺失(+)和缺失(0)等位基因分布频率研究。结果表明,与对照组相比,支气管哮喘患者GSTM1基因缺失的纯合子(0/0)频率(81 2%)显著升高(χ2=32 46,P<0 001;wχ2=28 75,P<0 001)。对于GSTT1也得到类似资料。而支气管哮喘患者GSTT1基因缺失等位基因(0/0)频率(71 7%)比对照组(11 7%)显著升高(χ2=26 72,P<0 001;wχ2=35 75,P<0 001)。表明GSTM1、GSTT1缺失等位基因纯合性在哮喘患者中是最有特征性的。GSTM10/0、GSTT10/0结合的频率患者组为61 7%,对照组仅为1 7%(χ2=27 3,P<0 001)。提示GSTM1和GSTT1基因多态性与哮喘有显著性关联,两个基因的突变可以被视为发生支气管哮喘遗传风险因子。To study the association of genes polymorphisms in glutathione S-transferase M1 and T1 with asthma bronchial. The distribute frequency of allele (+) and allele (0) between GSTM1 and GSTT1 of 60 patients asthma bronchial and 60 control groups in Tangshan was studied with PCR. The result shown GSTM1 deficiency allele(0/0) frequency of asthma bronchial was 81.2%, which showed significantly higher(χ~2=32.46, P<0.001; wχ~2=28.75, P<0.001) than the control groups; GSTT1 was similar to GSTM1. But GSTT1 zero allele(0/0) frequency of asthma bronchial were 71.7%, which were significantly higher (χ~2=26.72, P<0.001; wχ~2=35.75, P<0.001) than the control groups(11.7%). Zero allele of GSTM1 and GSTT1 were showed the most features in the asthma bronchial. Associated significantly in the genes polymorphisms of GSTM1 and GSTT1 with asthma bronchial, their genes mutation may be the genetic risk factor of asthma bronchial.

关 键 词：支气管哮喘 遗传因子 遗传多态性 谷胱甘肽-S-转移酶基因 

分 类 号：R562.2[医药卫生—呼吸系统] R394[医药卫生—内科学]