水稻线粒体基因的表达受核背景的影响  

Genomic Context of Nucleus Influences Mitochondrial Gene Expression of HL-type Rice

作　　者：易平[1] 余涛[2] 刘义[1] 朱英国[2] 

机构地区：[1]武汉大学化学与分子科学学院,武汉430072 [2]武汉大学植物发育生物学教育部重点实验室,武汉430072

出　　处：《遗传》2004年第2期186-188,共3页Hereditas（Beijing)

基　　金：国家973项目(2001CB108806)~~]

摘　　要：高等植物细胞核与线粒体之间的互作机理一直是发育生物学的研究热点,而普遍存在于高等植物中的细胞质雄性不育现象的发现,为该领域的研究提供了极好的材料。以红莲型细胞质雄性不育系A、保持系B和两种杂交一代(F1和SF1)为材料,利用一种适用于线粒体基因表达分析的差异展示方法,研究了不同核背景下线粒体的变化情况。结果表明,核背景的改变对线粒体基因的表达具有广泛的影响。In eukaryotic cells,the role of mitochondria is to generate both ATP and carbon skeletons for biosynthetic purposes.Meanwhile,the mitochondrial genes,in plant,are also involved in the development of anther.The presence of genetic information within mitochondria and nucleus make intracellular cooperation necessary.In this paper,a new RAPD method is used to analyze the expression differences of rice mitochondrial gene with the change of nuclear background.Many different fragments are obtained from hybrids,but not from sterile line or remainder line.According to the results,it is concluded that there are extensive effects of nuclear background on mitochondrial gene expression.

关 键 词：水稻 线粒体 基因 表达 细胞质雄性不育 

分 类 号：S511[农业科学—作物学]